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Evaluation of cytotoxic and genotoxic effects of functionalized nanocellulose in a co-culture system approaching the lung environment

2021-04-20Conference object Restricted access
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dc.contributor.authorPinto, Fátima
dc.contributor.authorVentura, Célia
dc.contributor.authorLourenço, Ana Filipa
dc.contributor.authorFerreira, Paulo J.T.
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-01-14T14:51:56Z
dc.date.available2022-01-14T14:51:56Z
dc.date.issued2021-04-20
dc.description.abstractIntroduction: Nanocelluloses, obtained from different sources and by various methods and holding different functionalization are innovative environmentally friendly materials in both pure and composite forms that hold great promise for industrial or advanced biomedical applications. Based on previous knowledge on other nanofibers e.g., carbon nanotubes, revealing toxicity at cellular and organismal levels, there is a need to evaluate the toxic potential of new nanocelluloses, particularly cellulose nanofibrils (CNF), before entering the market. In this work, three different types of nanocelluloses produced from Eucalyptus globulus bleached kraft pulp but differing in important physicochemical properties such as the carboxyl content (CCOOH), degree of polymerization (DP), morphology, specific surface area and size of fibrils, were investigated regarding cyto- and genotoxic effects in human alveolar cells and in a co-culture of human alveolar cells (A549 cells) and THP-1 differentiated macrophages. Results: A preliminary evaluation of the cytotoxicity in A549 by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that all nanocellulose samples showed no toxicity over the examined concentration range during a 24h exposure period. The genotoxicity of the nanocellulose samples was assessed through the cytokinesis-blocked micronucleus assay in A549 co-cultured with THP-1 cells and exposed to 1.5, 3, 6, 12.5, and 25 μg/cm2 of each nanocellulose for 24h. Preliminary results showed no alterations in the frequency of micronucleated binuceated cells for all tested concentrations in one sample of CNF produced with a catalytic oxidation with TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) radical (T1300). Moreover, the cytokinesis-block proliferation index of exposed A549 cells did not show differences as compared with non-exposed cells, in line with the MTT results, suggesting that the tested nanocelluloses do not affect A549 cells proliferation. Conclusions: Preliminary results suggest that T1300 sample do not present cytotoxicity or genotoxicity in the examined concentration range and exposure time, as compared to controls. The other two samples are still being investigated.pt_PT
dc.description.abstractSummary: Three nanocellulose samples were investigated for their in vitro toxicity. No major cytotoxic or genotoxic effects were detected in alveolar cells co-cultured with THP-1 macrophages.pt_PT
dc.description.sponsorshipThe work was funded by FCT/MCTES through national funds (PIDDAC), project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017), UIDB/00102/2020 and UIDB/00009/2020; UIDP/00009/2020. The authors acknowledge CYTED network NANOCELIA (Transferencia Tecnológica sobre aplicaciones de nanocelulosa en iberoamerica)pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7851
dc.language.isoengpt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectNanocellulosept_PT
dc.subjectCo-culturept_PT
dc.subjectLung Cellspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleEvaluation of cytotoxic and genotoxic effects of functionalized nanocellulose in a co-culture system approaching the lung environmentpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title10th International Conference on Nanotoxicology (NANOTOX), 20-22 april 2021pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVentura
person.familyNameLouro
person.familyNameSilva
person.givenNameCélia
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier2056460
person.identifier157627
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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