Conventional and Novel ‘Omics’-Based Approaches to the Study of Carbon Nanotubes Pulmonary Toxicity

Ventura, Célia; Sousa-Uva, António; Lavinha, João; Silva, Maria João

Publication

Conventional and Novel ‘Omics’-Based Approaches to the Study of Carbon Nanotubes Pulmonary Toxicity

2018-05Journal article

dc.contributor.author	Ventura, Célia
dc.contributor.author	Sousa-Uva, António
dc.contributor.author	Lavinha, João
dc.contributor.author	Silva, Maria João
dc.date.accessioned	2019-02-19T17:30:46Z
dc.date.available	2019-02-19T17:30:46Z
dc.date.issued	2018-05
dc.description.abstract	The widespread application of carbon nanotubes (CNT) on industrial, biomedical, and consumer products can represent an emerging respiratory occupational hazard. Particularly, their similarity with the fiber-like shape of asbestos have raised a strong concern about their carcinogenic potential. Several in vitro and in vivo studies have been supporting this view by pointing to immunotoxic, cytotoxic and genotoxic effects of some CNT that may conduct to pulmonary inflammation, fibrosis, and bronchioloalveolar hyperplasia in rodents. Recently, high throughput molecular methodologies have been applied to obtain more insightful information on CNT toxicity, through the identification of the affected biological and molecular pathways. Toxicogenomic approaches are expected to identify unique gene expression profiles that, besides providing mechanistic information and guiding new research, have also the potentialto be used as biomarkers for biomonitoring purposes. In this review, the potential of genomic data analysis is illustrated by gene network and gene ontology enrichment analysis of a set of 41 differentially expressed genes selected from a literature search focused on studies of C57BL/6 mice exposed to the multiwalled CNT Mitsui-7. The majority of the biological processes annotated in the network are regulatory processes and the molecular functions are related to receptor-binding signalling. Accordingly, the network-annotated pathways are cell receptor-induced pathways. A single enriched molecular function and one biological process were identified. The relevance of specific epigenomic effects triggered by CNT exposure, for example, alteration of the miRNA expression profile is also discussed in light of its use as biomarkers in occupational health studies.	pt_PT
dc.description.sponsorship	Centre for Toxicogenomics and Human Health (ToxOmics); Foundation for Science and Technology (UID/BIM/00009/2013)	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Environ Mol Mutagen. 2018 May;59(4):334-362. doi: 10.1002/em.22177. Epub 2018 Feb 26	pt_PT
dc.identifier.doi	10.1002/em.2217	pt_PT
dc.identifier.issn	0893-6692
dc.identifier.uri	http://hdl.handle.net/10400.18/5908
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Environmental Mutagen Society
dc.relation.publisherversion	https://onlinelibrary.wiley.com/doi/full/10.1002/em.22177	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Toxicogenomics	pt_PT
dc.subject	Toxicoepigenomics	pt_PT
dc.subject	Carbon Nanotubes	pt_PT
dc.subject	Gene Expression	pt_PT
dc.subject	miRNA	pt_PT
dc.subject	Nanotoxicology	pt_PT
dc.subject	Genómica Funcional e Estrutural	pt_PT
dc.title	Conventional and Novel ‘Omics’-Based Approaches to the Study of Carbon Nanotubes Pulmonary Toxicity	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.endPage	362	pt_PT
oaire.citation.issue	4	pt_PT
oaire.citation.startPage	334	pt_PT
oaire.citation.title	Environmental and Molecular Mutagenesis	pt_PT
oaire.citation.volume	59	pt_PT
oaire.fundingStream	5876
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	e9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscovery	e9cc9728-4f09-4e3a-b30d-53d4429986fb