Repository logo
 
Publication

Rhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia ovis Infection: Identification of Candidate Protective Antigens

2018-05-04Journal article Open access
Simple item page
dc.contributor.authorAntunes, S.
dc.contributor.authorCouto, J.
dc.contributor.authorFerrolho, J.
dc.contributor.authorRodrigues, F.
dc.contributor.authorNobre, J.
dc.contributor.authorSantos, A.S.
dc.contributor.authorSantos-Silva, M.M.
dc.contributor.authorde la Fuente, J.
dc.contributor.authorDomingos, A.
dc.date.accessioned2018-11-19T16:39:13Z
dc.date.available2018-11-19T16:39:13Z
dc.date.issued2018-05-04
dc.description.abstractTicks are among the most prevalent blood-feeding arthropods, and they act as vectors and reservoirs for numerous pathogens. Sialotranscriptomic characterizations of tick responses to blood feeding and pathogen infections can offer new insights into the molecular interplay occurring at the tick-host-pathogen interface. In the present study, we aimed to identify and characterize Rhipicephalus bursa salivary gland (SG) genes that were differentially expressed in response to blood feeding and Babesia ovis infection. Our experimental approach consisted of RNA sequencing of SG from three different tick samples, fed-infected, fed-uninfected, and unfed-uninfected, for characterization and inter-comparison. Overall, 7,272 expressed sequence tags (ESTs) were constructed from unfed-uninfected, 13,819 ESTs from fed-uninfected, and 15,292 ESTs from fed-infected ticks. Two catalogs of transcripts that were differentially expressed in response to blood feeding and B. ovis infection were produced. Four genes coding for a putative vitellogenin-3, lachesin, a glycine rich protein, and a secreted cement protein were selected for RNA interference functional studies. A reduction of 92, 65, and 51% was observed in vitellogenin-3, secreted cement, and lachesin mRNA levels in SG, respectively. The vitellogenin-3 knockdown led to increased tick mortality, with 77% of ticks dying post-infestation. The reduction of the secreted cement protein-mRNA levels resulted in 46% of ticks being incapable of correctly attaching to the host and significantly lower female weights post-feeding in comparison to the control group. The lachesin knockdown resulted in a 70% reduction of the levels associated with B. ovis infection in R. bursa SG and 70% mortality. These results improved our understanding of the role of tick SG genes in Babesia infection/proliferation and tick feeding. Moreover, lachesin, vitellogenin-3, and secreted cement proteins were validated as candidate protective antigens for the development of novel tick and tick-borne disease control measures.pt_PT
dc.description.sponsorshipFCT for funds to GHTM UID/Multi/04413/2013.
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Cell Infect Microbiol. 2018 May 4;8:116. doi: 10.3389/fcimb.2018.00116. eCollection 2018pt_PT
dc.identifier.doi10.3389/fcimb.2018.00116. eCollection 2018pt_PT
dc.identifier.issn2235-2988
dc.identifier.urihttp://hdl.handle.net/10400.18/5661
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relation.publisherversionhttps://doi.org/10.3389/fcimb.2018.00116pt_PT
dc.subjectSialotranscriptomicspt_PT
dc.subjectRhipicephalus bursapt_PT
dc.subjectBabesia spp.pt_PT
dc.subjectRNA Interferencept_PT
dc.subjectvaccinept_PT
dc.subjectvector-pathogen Interactionspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleRhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia ovis Infection: Identification of Candidate Protective Antigenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage116pt_PT
oaire.citation.startPage116pt_PT
oaire.citation.titleFrontiers in Cellular and Infection Microbiologypt_PT
oaire.citation.volume8pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
230 ASantos, MMSantos-Silva Rhipicephalus bursa... Frontiers.pdf
Size:
1.94 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais