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Differentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestos

2020-09-12Conference object Restricted access
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dc.contributor.authorVentura, Célia
dc.contributor.authorVieira, Luís
dc.contributor.authorSilva, Catarina
dc.contributor.authorSilva, Maria João
dc.date.accessioned2021-03-13T15:45:30Z
dc.date.available2021-03-13T15:45:30Z
dc.date.issued2020-09-12
dc.description.abstractEnvironmental and occupational exposure to multi-walled carbon nanotubes (MWCNT), an engineered nanomaterial widely used in industrial and biomedical applications, has been associated with pulmonary adverse effects upon inhalation. Particularly, MWCNT-7 (Mitsui-7) has been classified as possibly carcinogenic to humans, and many toxicological studies have compared it with asbestos due to their similarity in physical characteristics and biopersistence, and the well-known pulmonary adverse effects of asbestos. Several pulmonary diseases have been associated with miRNA expression changes, thus, in this study, we compare the differentially expressed miRNAs (DEmiRNAs) and enriched KEGG pathways after 1 and 7 days exposure to MWCNT-7 or crocidolite, in order to understand the mechanisms of biological response to these materials over time and their possible similarity. For this purpose, miRNAs were sequenced in exposed and non-exposed A549 cells at both time points by next-generation sequencing, and the enriched cellular pathways identified as previously described with minor modifications1. A higher number of DEmiRNAs was observed at 24 h compared to 7 days (17 vs. 3 and 21 vs. 10 for MWCNT-7 and crocidolite, respectively). Accordingly, the number of enriched pathways was also higher at 24 h (23 vs. 30 at 24 h and 7 vs. 5 at 7 days for MWCNT-7 and crocidolite, respectively). At 7 days, most altered pathways were related to cancer, highlighting the carcinogenic potential of MWCNT-7 and crocidolite. The reduction of the number of affected functional pathways likely reflects a greatest impact of these materials shortly after exposure and/or that the differences in DEmiRNAs between non-exposed and exposed cells are less significant after long-term culture. 1Ventura et al, Toxicology Letters 328: 7-18pt_PT
dc.description.sponsorshipFCT/MCTES: Project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017) through national funds (PIDDAC) and ToxOmics (UIDP/00009/2020). GenomePT project (POCI-01- 0145-FEDER-022184), supported by COMPETE 2020 - POCI, Lisboa2020, CRESC Algarve2020, through the ERDF, and by FCT.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7450
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectNanomaterialspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectmiRNApt_PT
dc.subjectMultiwalled carbon nanotubespt_PT
dc.subjectNanotoxicologypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectAsbestospt_PT
dc.titleDifferentially Expressed miRNAs and Functional Pathways Changes in Human Alveolar Epithelial Cells After 1 and 7 Days Exposure to MWCNT-7 or Asbestospt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.titleEnvironmental Mutagenesis & Genomics Society Virtual Annual Meeting (EMGS), 12-16 September 2020pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVentura
person.familyNameSilva
person.givenNameCélia
person.givenNameMaria Joao
person.identifier2056460
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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