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Hazard Assessment of Benchmark Metal-Based Nanomaterials Through a Set of In Vitro Genotoxicity Assays

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dc.contributor.authorVital, Nádia
dc.contributor.authorPinhão, Mariana
dc.contributor.authorYamani, Naouale El
dc.contributor.authorRundén-Pran, Elise
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorDušinská, Maria
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-11-29T11:24:52Z
dc.date.available2022-11-29T11:24:52Z
dc.date.issued2022
dc.descriptionErratum: Adv Exp Med Biol. 2022;1357:C1. doi: 10.1007/978-3-030-88071-2_18. The original version of this chapter was revised: The reference citations within the text weren’t updated in sequential order. It has been corrected now; the renumbered citations have been updated throughout the respective chapter.
dc.description.abstractFor safety assessment of nanomaterials (NMs), in vitro genotoxicity data based on welldesigned experiments is required. Metal-based NMs are amongst the most used in consumer products. In this chapter, we report results for three metal-based NMs, titanium dioxide (NM- 100), cerium dioxide (NM-212) and silver (NM-302) in V79 cells, using a set of in vitro genotoxicity assays covering different endpoints: the medium-throughput comet assay and its modified version (with the enzyme formamidopyrimidine DNA glycosylase, Fpg), measuring DNA strand beaks (SBs) and oxidized purines, respectively; the micronucleus (MN) assay, assessing chromosomal damage; and the Hprt gene mutation test. The results generated by this test battery showed that all NMs displayed genotoxic potential. NM-100 induced DNA breaks, DNA oxidation damage and point mutations but not chromosome instability. NM-212 increased the level of DNA oxidation damage, point mutations and increased the MN frequency at the highest concentration tested. NM-302 was moderately cytotoxic and induced gene mutations, but not DNA or chromosome damage. In conclusion, the presented in vitro genotoxicity testing strategy allowed the identification of genotoxic effects caused by three different metal-based NMs, raising concern as to their impact on human health. The results support the use of this in vitro test battery for the genotoxicity assessment of NMs, reducing the use of more expensive, time-consuming and ethically demanding in vivo assays, in compliance with the 3 R’s.pt_PT
dc.description.sponsorshipThis research was co-funded by the EC FP7 NANoREG (Grant Agreement NMP4-LA-2013–310584), the EC QualityNano Research Infrastructure project (Grant Agreement No: INFRA-2010-262163), through the QualityNano Transnational Access fellowships [NILU-TAF-410 and NILU-TAF-403] attributed to N. Vital and M. J. Silva and by the Portuguese Foundation for Science and Technology through ToxOmics (UIDB/00009/2020; UIDP/00009/2020). N. Vital work is also supported by the Portuguese Foundation for Science and Technology PhD Scholarship 2020.07168.BD.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationIn: Louro H, Silva MJ (eds). Nanotoxicology in Safety Assessment of Nanomaterials. Springer, Cham., 2022, pp 351-75. (Advances in Experimental Medicine and Biology;1357). https://doi.org/10.1007/978-3-030-88071-2_14pt_PT
dc.identifier.doi10.1007/978-3-030-88071-2_14pt_PT
dc.identifier.eissn2214-8019
dc.identifier.issn0065-2598
dc.identifier.urihttp://hdl.handle.net/10400.18/8363
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationA common European approach to the regulatory testing of nanomaterials
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relationA pan-European infrastructure for quality in nanomaterials safety testing
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.relation.publisherversionhttps://link.springer.com/chapter/10.1007/978-3-030-88071-2_14#citeaspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectNanomaterialspt_PT
dc.subjectDNA Damagept_PT
dc.subjectGenotoxicitypt_PT
dc.subjectMutagenicitypt_PT
dc.subjectSilver Nanorods/wirespt_PT
dc.subjectCerium Dioxidept_PT
dc.subjectTitanium Dioxidept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleHazard Assessment of Benchmark Metal-Based Nanomaterials Through a Set of In Vitro Genotoxicity Assayspt_PT
dc.title.alternativeNanotoxicology in Safety Assessment of Nanomaterialspt_PT
dc.typebook part
dspace.entity.typePublication
oaire.awardTitleA common European approach to the regulatory testing of nanomaterials
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleA pan-European infrastructure for quality in nanomaterials safety testing
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/310584/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/262163/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.citation.endPage375pt_PT
oaire.citation.startPage351pt_PT
oaire.citation.titleAdvances in Experimental Medicine and Biologypt_PT
oaire.citation.volume(Advances in Experimental Medicine and Biology;1357)pt_PT
oaire.fundingStreamFP7
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamFP7
person.familyNameVital
person.familyNameLouro
person.familyNameSilva
person.givenNameNádia
person.givenNameHenriqueta
person.givenNameMaria João
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-3391-0268
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0001-8821-0747
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
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