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Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri

2021-07-19Journal article Open access
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dc.contributor.authorBonifácio, Marina
dc.contributor.authorMateus, Cristiana
dc.contributor.authorAlves, Ana R.
dc.contributor.authorMaldonado, Emanuel
dc.contributor.authorDuarte, Ana P.
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.contributor.authorFerreira, Susana
dc.date.accessioned2022-07-03T16:03:16Z
dc.date.available2022-07-03T16:03:16Z
dc.date.issued2021-07-19
dc.description(This article belongs to the Collection New Insights into Bacterial Pathogenesis)pt_PT
dc.description.abstractAliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments. Whether natural transformation can be a mechanism that generates genetic diversity in A. butzleri is still unknown. In the present study, we aimed to establish if A. butzleri is naturally competent for transformation and to investigate the factors influencing this process. Two different transformation procedures were tested using exogenous and isogenic DNA containing antibiotic resistance markers, and different external conditions influencing the process were evaluated. The highest number of transformable A. butzleri strains were obtained with the agar transformation method when compared to the biphasic system (65% versus 47%). A. butzleri was able to uptake isogenic chromosomal DNA at different growth phases, and the competence state was maintained from the exponential to the stationary phases. Overall, the optimal conditions for transformation with the biphasic system were the use of 1 μg of isogenic DNA and incubation at 30 °C under a microaerobic atmosphere, resulting in a transformation frequency ~8 × 10-6 transformants/CFU. We also observed that A. butzleri favored the transformation with the genetic material of its own strain/species, with the DNA incorporation process occurring promptly after the addition of genomic material. In addition, we observed that A. butzleri strains could exchange genetic material in co-culture assays. The presence of homologs of well-known genes involved in the competence in the A. butzleri genome corroborates the natural competence of this species. In conclusion, our results show that A. butzleri is a naturally transformable species, suggesting that horizontal gene transfer mediated by natural transformation is one of the processes contributing to its genetic diversity. In addition, natural transformation can be used as a tool for genetic studies of this species.pt_PT
dc.description.sponsorshipThis work was supported by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB/00709/2020. This work was also funded by the operation CENTRO-01-0145-FEDER-000019 – C4 – Centro de Competências em Cloud Computing, supported by the European Regional Development Fund (ERDF) through the Regional Operational Program of the Center (Centro2020). Cristiana Mateus is the recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020). Susana Ferreira acknowledges the Universidade da Beira Interior and FCT by the contract of Scientific Employment according to DL57/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPathogens. 2021 Jul 19;10(7):909. doi: 10.3390/pathogens10070909pt_PT
dc.identifier.doi10.3390/pathogens10070909pt_PT
dc.identifier.issn2076-0817
dc.identifier.urihttp://hdl.handle.net/10400.18/8051
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationHealth Sciences Research Centre
dc.relationUnravelling the virulence potential of Aliarcobacter butzleri
dc.relationHealth Sciences Research Centre
dc.relationNot Available
dc.relation.publisherversionhttps://www.mdpi.com/2076-0817/10/7/909pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAliarcobacter butzleript_PT
dc.subjectNatural Competencept_PT
dc.subjectTransformationpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleNatural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleUnravelling the virulence potential of Aliarcobacter butzleri
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F151023%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL57%2F2016%2FCP1334%2FCP1644%2FCT0001/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage909pt_PT
oaire.citation.titlePathogenspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublication60f6a70b-110d-4c15-a3fd-d43d078078bc
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