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Development of a new multiplex PCR to detect prevalent species of house dust mites in house dust

2022-07Journal article Restricted access
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dc.contributor.authorOliveira, Ana Sofia
dc.contributor.authorGaspar, Carlos
dc.contributor.authorRolo, Joana
dc.contributor.authorPereira, Cristiana Costa
dc.contributor.authorPalmeira-de-Oliveira, Rita
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorMartinez-de-Oliveira, José
dc.contributor.authorPalmeira-de-Oliveira, Ana
dc.date.accessioned2023-03-22T11:28:16Z
dc.date.available2023-03-22T11:28:16Z
dc.date.issued2022-07
dc.description.abstractDermatophagoides pteronyssinus and Dermatophagoides farinae are the most common House Dust Mite (HDM) species in home environments worldwide and responsible for HDM allergy. Since the prevalence of HDM-related clinical conditions is linked to exposure to the mite itself, the detection of HDM in the human households gains importance. We aimed to develop a fast and accessible multiplex PCR to detect and distinguish two relevant HDM species in house dust. New primers were designed, and sensitivity analysis was performed. Sequencing of PCR products was also performed to confirm the method's specificity. The limit of detection of the multiplex PCR for both species was as low as 30 pg µL-1. The application of the multiplex PCR to dust samples also resulted in the identification of both species with high sensitivity. The protocol required small amount of template, reagents and a short reaction time thus presenting an alternative to classically used techniques for HDM identification.pt_PT
dc.description.sponsorshipThis work was supported by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the Grant [UIDB/00709/2020]. Financial support was also provided by the Foundation for Science and Technology (FCT) through a PhD fellowship to A.S.O under Grant [SFRH//BD/136192/2018].pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Environ Health Res. 2022 Jul;32(7):1500-1512. doi: 10.1080/09603123.2021.1893282. Epub 2021 Mar 8.pt_PT
dc.identifier.doi10.1080/09603123.2021.1893282pt_PT
dc.identifier.issn0960-3123
dc.identifier.urihttp://hdl.handle.net/10400.18/8598
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor and Francispt_PT
dc.relationHealth Sciences Research Centre
dc.relationalterado para: “Portuguese Natural Resources as Modulators of Acne Vulgaris Hallmarks” Nonspecific vulvovaginitis: the putative ethiopatologic role of House Dust Mites
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/09603123.2021.1893282pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDermatophagoidespt_PT
dc.subjectDustpt_PT
dc.subjectMultiplex PCRpt_PT
dc.subjectHouse Dust Mitept_PT
dc.subjectAllergenspt_PT
dc.subjectAr e Saúde Ocupacionalpt_PT
dc.titleDevelopment of a new multiplex PCR to detect prevalent species of house dust mites in house dustpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitlealterado para: “Portuguese Natural Resources as Modulators of Acne Vulgaris Hallmarks” Nonspecific vulvovaginitis: the putative ethiopatologic role of House Dust Mites
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F136192%2F2018/PT
oaire.citation.endPage1512pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1500pt_PT
oaire.citation.titleInternational Journal of Environmental Health Researchpt_PT
oaire.citation.volume32pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublication6bd83b99-9fe6-4caf-bd57-761c17810c25
relation.isProjectOfPublication.latestForDiscoveryced21566-cfde-4640-afff-0536a07c1f8f

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