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Methods of Extraction, Detection, and Identification of Different Metabolites-A Metabolomics Approach

2025-02-06Capítulo de livro Acesso restrito
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datacite.subject.fosCiências Naturais
dc.contributor.authorBennici, Giulia
dc.contributor.authorPermatasari, Devi
dc.contributor.authorAl-Younis, Inas
dc.contributor.authorDhahri, Manel
dc.contributor.authorPinto, Frederico G.
dc.contributor.authorUddin, Mohammed J.
dc.contributor.authorEmwas, Abdul-Hamid
dc.contributor.authorJaremko, Mariusz
dc.contributor.authorSoares, Nelson C.
dc.contributor.editorSoliman, Sameh S.M.
dc.contributor.editorHusseiny, Mohamed I.
dc.date.accessioned2026-03-06T16:47:14Z
dc.date.available2026-03-06T16:47:14Z
dc.date.issued2025-02-06
dc.description.abstractMetabolic dynamics in attack and defense mechanisms are fascinating topics that illustrate the complex ways organisms adapt to their environments. Attack mechanisms involve several functions, including energy production and metabolic adaptation, where some organisms change their appearance or behavior to balance into their environment. Defense mechanisms involve stress response, immune system activation, repair, and recovery. This chapter provides an overview of the methods applied in modern metabolomics, including single-cell metabolomics and targeted/untargeted approaches, emphasizing how metabolite production rapidly shifts in response to environmental and cellular changes. To effectively monitor these spontaneous effects, it is essential to conduct metabolomics studies with a carefully controlled experimental design. This includes meticulous sample collection, efficient metabolite extraction, and optimization of parameters for the most relevant analytical platform. This chapter outlines the most relevant and commonly used methods for metabolite extraction, aiming to maximize the number of metabolites recovered. This is followed by a summary of the advantages and limitations of the most frequently employed analytical tools, with a particular focus on nuclear magnetic resonance (NMR) and various types of mass spectrometry (MS). Finally, we emphasize the importance of integrating different omics sciences to achieve a comprehensive understanding of biological systems.eng
dc.identifier.citationIn: Soliman SSM, Husseiny MI (eds). Metabolic Dynamics in Host-Microbe Interaction. Singapore: Springer, 2025, pp. 33-39 (chapter 3). doi:10.1007/978-981-96-1305-2_3
dc.identifier.doi10.1007/978-981-96-1305-2_3
dc.identifier.isbn978-981-96-1305-2
dc.identifier.urihttp://hdl.handle.net/10400.18/11181
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relation.hasversionhttps://link.springer.com/chapter/10.1007/978-981-96-1305-2_3
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMetabolomics
dc.subjectMetabolite Extraction
dc.subjectMetabolite Detection
dc.subjectHost-Pathogen Interaction
dc.subjectSingle-Cell Metabolomics
dc.subjectGenómica Funcional e Estrutural
dc.titleMethods of Extraction, Detection, and Identification of Different Metabolites-A Metabolomics Approacheng
dc.typebook part
dspace.entity.typePublication
oaire.citation.endPage49
oaire.citation.startPage33
oaire.citation.titleMetabolic Dynamics in Host-Microbe Interaction
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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