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Levofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilms

2017-10-30Journal article Restricted access
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dc.contributor.authorFerreira, Magda
dc.contributor.authorRzhepishevska, Olena
dc.contributor.authorGrenho, Liliana
dc.contributor.authorMalheiros, Danila
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorAlmeida, António J.
dc.contributor.authorJordao, Luisa
dc.contributor.authorRibeiro, Isabel A.
dc.contributor.authorRamstedt, Madeleine
dc.contributor.authorGomes, Pedro
dc.contributor.authorBettencourt, Ana
dc.date.accessioned2018-02-14T12:28:54Z
dc.date.available2021-01-01T01:30:11Z
dc.date.issued2017-10-30
dc.description.abstractStaphylococcus aureus is a major pathogen in bone associated infections due to its ability to adhere and form biofilms on bone and/or implants. Moreover, recrudescent and chronic infections have been associated with S. aureus capacity to invade and persist within osteoblast cells. With the growing need of novel therapeutic tools, this research aimed to evaluate some important key biological properties of a novel carrier system composed of acrylic bone cement (polymethylmethacrylate - PMMA), loaded with a release modulator (lactose) and an antibiotic (levofloxacin). Levofloxacin-loaded bone cement (BC) exhibited antimicrobial effects against planktonic and biofilm forms of S. aureus (evaluated by a flow chamber system). Moreover, novel BC formulation showed high anti-bacterial intraosteoblast activity. This fact led to the conclusion that levofloxacin released from BC matrices could penetrate the cell membrane of osteoblasts and be active against S. aureus strains in the intracellular environment. Furthermore, levofloxacin-BC formulations showed no significant in vitro cytotoxicity and no allergic potential (measured by the in vivo chorioallantoic membrane assay). Our results indicate that levofloxacin-loaded BC has potential as a local antibiotic delivery system for treating S. aureus associated bone infections.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia from Portuguese government (Project PEst-OE/SAU/UI4013/2014). The paper is based upon the work from COST TD1305 (Improved Protection of Medical Devices against infection).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Pharm. 2017 Oct 30;532(1):241-248. doi: 10.1016/j.ijpharm.2017.08.089. Epub 2017 Aug 26.pt_PT
dc.identifier.doi10.1016/j.ijpharm.2017.08.089pt_PT
dc.identifier.issn0378-5173
dc.identifier.urihttp://hdl.handle.net/10400.18/4950
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://doi.org/10.1016/j.ijpharm.2017.08.089pt_PT
dc.subjectCementspt_PT
dc.subjectLevofloxacinpt_PT
dc.subjectBiofilmspt_PT
dc.subjectToxicitypt_PT
dc.subjectBiocompatibilitypt_PT
dc.subjectBone-infectionpt_PT
dc.subjectFlow-chamber Systempt_PT
dc.subjectFluoroquinolone-delivery-systempt_PT
dc.subjectOsteoblast-infection-modelpt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.titleLevofloxacin-loaded bone cement delivery system: highly effective against intracellular bacteria and Staphylococcus aureus biofilmspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FDTP%2F04138%2F2013/PT
oaire.citation.conferencePlaceAmsterdam, The Netherlandspt_PT
oaire.citation.endPage248pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage241pt_PT
oaire.citation.titleInternational Journal of Pharmaceuticspt_PT
oaire.citation.volume532pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctDe acordo com a política da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication8b7dc2e1-c04d-4a11-8fa9-83ead9d8f256
relation.isProjectOfPublication.latestForDiscovery8b7dc2e1-c04d-4a11-8fa9-83ead9d8f256

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