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Epidemiology and molecular characterization of invasive disease in children twenty years after the implementation of Haemophilus influenzae serotype b vaccine in Portuguese Immunization Programme

2021-07-12Conference object Open access
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dc.contributor.authorBajanca-Lavado, Maria Paula
dc.contributor.authorBettencout, Célia
dc.contributor.authorCunha, Florbela
dc.contributor.authorGonçalo-Marques, José
dc.contributor.authorStudy Group of invasive Haemophilus influenzae disease in of the Pediatric Infection Disease Society
dc.date.accessioned2022-07-10T17:40:54Z
dc.date.available2022-07-10T17:40:54Z
dc.date.issued2021-07-12
dc.description.abstractBackground: Haemophilus influenzae is an important human pathogen responsible for severe childhood invasive disease, despite the implementation of the vaccine against serotype b isolates (Hib), in our National Immunization Programme (NIP), in June 2000. The use of the vaccine lead to a reduction in Hib invasive disease, together with the emergence of non-encapsulated (NTHi), and capsulated non-b-type isolates. This study aims to characterize H. influenzae invasive disease in children, twenty years after the introduction of the Hib vaccine in NIP. Methods Hundred-twenty invasive H. influenzae isolates collected from children in 33 Hospitals, between January 2010 and December 2020, were characterized at the National Reference Laboratory for Haemophilus influenzae. Antibiotic susceptibility was assessed by a microdilution assay. Capsular status was identified by PCR as previously described. MLST was performed as described in the literature. Sequences were analysed and submitted to the MLST website (https://pubmlst.org/hinfluenzae/) for assignment of the sequence type (ST). goeBURST analysis was performed using the PHYLOViZ platform. Results Childhood invasive disease was mainly due to NTHi (55.8%; 67/120), although Hib still in circulation (29.2%; 35/120). Twenty-two cases of vaccine failures were responsible for 62.9% of Hib disease, with 59% of cases occurring in last four years. Non-b capsular types isolates were distributed as follow: 9.2% serotype a (11/120), 1.6% serotype e (2/120) and 4.2% serotype f (5/120). Most isolates were susceptible to all antibiotics studied, with 8.3% (10/120) being ampicillin resistant by β-lactamase producing. MLST revealed, as expected, high genetic variability (77.1%), with 37 different STs among 48 NTHi isolates. In opposition, encapsulated isolates were clonal with Hia assigned to CC23 (ST23-n=6; ST1511-n=1), Hib to CC6 (ST6-n=27, ST190, ST1149 and ST1231 with one isolate each), Hie to CC18 (ST18-n=2) and Hif to CC124 (ST124-n=2, ST1188-n=1). Conclusions Our data suggests that after vaccine implementation, invasive disease among Portuguese children is mainly due to highly genetically diverse, susceptible NTHi isolates. Nevertheless, we are concerned about Hib disease (~30%) despite the higher vaccine coverage observed in our country. Ongoing surveillance should be continued, in order to monitor the burden of the disease, especially Hib, and develop additional public health prevention strategies.pt_PT
dc.description.sponsorshipThis study was totally financed by National Institute of Health, Portugalpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMaria Paula Bajanca-Lavado, Célia Bettencourt, Florbela Cunha, José Gonçalo-Marques, on behalf of Study Group of invasive Haemophilus influenzae Disease of the Pediatric Infection Disease Society. Epidemiology and molecular characterization of invasive disease in children twenty years after the implementation of Haemophilus influenzae serotype b vaccine in Portuguese Immunization Programme. Comunicação oral (01004) apresentada na sessão “Epidemiology of Vaccine Preventable Diseases”, no 31st ECCMID (Online 9-12 July 2021).pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8203
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectH. influenzae Invasive Diseasept_PT
dc.subjectChildrenpt_PT
dc.subjectMultilocus Sequencing Typingpt_PT
dc.subjectSerotypingpt_PT
dc.subjectVaccine Failurept_PT
dc.subjectHaemophilus influenzaept_PT
dc.subjectEpidemiology Characterizationpt_PT
dc.subjectMolecular Characterizationpt_PT
dc.subjectDoenças Evitáveis pela Vacinaçãopt_PT
dc.subjectInfecções Respiratórias
dc.subjectPortugal
dc.titleEpidemiology and molecular characterization of invasive disease in children twenty years after the implementation of Haemophilus influenzae serotype b vaccine in Portuguese Immunization Programmept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), 9-12 July 2021pt_PT
person.familyNameBajanca Lavado
person.givenNameMaria Paula
person.identifier.ciencia-id2B11-E245-3D39
person.identifier.orcid0000-0002-1453-0315
person.identifier.scopus-author-id6506614938
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication31093b71-e0a5-4796-8b01-12f4eccfc57a
relation.isAuthorOfPublication.latestForDiscovery31093b71-e0a5-4796-8b01-12f4eccfc57a

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