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Molecular profiling of the human nasal epithelium: a proteomics approach

2011-05-18Journal article Restricted access
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dc.contributor.authorSimoes, Tania
dc.contributor.authorCharro, Nuno
dc.contributor.authorBlonder, Josip
dc.contributor.authorFaria, Daniel
dc.contributor.authorCouto, Francisco
dc.contributor.authorChan, King C.
dc.contributor.authorWaybright, Timothy
dc.contributor.authorIsaaq, Haleem J.
dc.contributor.authorVeenstra, Timothy D.
dc.contributor.authorPenque, Deborah
dc.date.accessioned2011-10-12T14:59:08Z
dc.date.available2011-10-12T14:59:08Z
dc.date.issued2011-05-18
dc.description.abstractA comprehensive proteomic profiling of nasal epithelium (NE) is described. This study relies on simple subcellular fractionation used to obtain soluble- and membrane-enriched fractions followed by 2-dimensional liquid chromatography (2D-LC) separation and tandem mass spectrometry (MS/MS). The cells were collected using a brushing technique applied on NE of clinically evaluated volunteers. Subsequently, the soluble- and the membrane-protein enriched fractions were prepared and analyzed in parallel using 2D-LC-MS/MS. In a set of 1482 identified proteins, 947 (63.9%) proteins were found to be associated to membrane fraction. Grand average hydropathy value index (GRAVY) analysis, the transmembrane protein mapping and annotations of primary location deposited in the Human Protein Reference Database (HPRD) confirmed an enrichment of hydrophobic proteins on this dataset. Ingenuity Pathway Analysis (IPA) of soluble fraction revealed an enrichment of molecular and cellular functions associated with cell death, protein folding and drug metabolism while in membrane fraction showed an enrichment of functions associated with molecular transport, protein trafficking and cell-to-cell signaling and interaction. The IPA showed similar enrichment of functions associated with cellular growth and proliferation in both soluble and membrane subproteomes. This finding was in agreement with protein content analysis using exponentially modified protein abundance index (emPAI). A comparison of our data with previously published studies focusing on respiratory tract epithelium revealed similarities related to identification of proteins associated with physical barrier function and immunological defence. In summary, we extended the NE molecular profile by identifying and characterizing proteins associated to pivotal functions of a respiratory epithelium, including the control of fluid volume and ionic composition at the airways' surface, physical barrier maintenance, detoxification and immunological defence. The extent of similarities supports the applicability of a less invasive analysis of NE to assess prognosis and treatment response of lung diseases such as asthma, cystic fibrosis and chronic obstructive pulmonary disease.por
dc.identifier.citationJ Proteomics. 2011 May 18. [Epub ahead of print]por
dc.identifier.otherESSN: 1876-7737
dc.identifier.otherdoi:10.1016/j.jprot.2011.05.012
dc.identifier.urihttp://hdl.handle.net/10400.18/295
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://linkinghub.elsevier.com/retrieve/pii/S1874-3919(11)00203-Xpor
dc.subjectNasal epitheliumpor
dc.subjectUpper and lower airwayspor
dc.subjectGenómica Funcional e Estruturalpor
dc.titleMolecular profiling of the human nasal epithelium: a proteomics approachpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Proteomicspor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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