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Mycotoxins and applicability of in vitro methods of digestion and absorption assessment - the patulin case study

2014-03Conference object Restricted access
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dc.contributor.authorAssunção, Ricardo
dc.contributor.authorFerreira, Mariana
dc.contributor.authorMartins, Carla
dc.contributor.authorDupont, Didier
dc.contributor.authorAlvito, Paula
dc.date.accessioned2014-05-30T12:36:23Z
dc.date.available2014-05-30T12:36:23Z
dc.date.issued2014-03
dc.description.abstractMycotoxins are a wide group of fungal secondary metabolites that exert multiple toxic effects on humans and animals1. Patulin, a mycotoxin with significant public health risk, is a toxic secondary metabolite produced by a wide range of fungal species growing on rotten fruit2. In human health risk assessment, ingestion of food is considered a major route of exposure to many contaminants, although the total amount of an ingested contaminant does not always reflect the amount that is available to the body and exert its toxic effects3. In this study, two in vitro methods were applied to evaluate the bioaccessibility of patulin at different experimental conditions and the intestinal membrane integrity of Caco-2 cells exposed to patulin and cysteine (antioxidant) protective effects. Seven artificially contaminated fruit juices were assayed in the presence or absence of a standard meal showing a significant difference (p=0.001) for bioaccessibility values between contaminated samples alone and combinations with a standard meal4. Different concentrations of patulin and cysteine were assayed in a Caco-2 cells monolayers. At 95 µM, patulin produced a dramatic decrease of transepithelial electrical resistance. This effect was significantly (p=0.016) reduced when 400 µM and 4000 µM of cysteine was added to the cells4. The combination of in vitro digestion models with other techniques using intestinal cell lines offer a more complete picture of what is happening in the digestion and absorption process, as well as the study of beneficial effects of protective agents.por
dc.description.sponsorshipFundação para a Ciência e Tecnologiapor
dc.identifier.citationProceedings of the 3rd Internatioal Conference on Food Digestion, 2014, p. 83por
dc.identifier.urihttp://hdl.handle.net/10400.18/2281
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCOST Action FA1005 INFOGESTpor
dc.subjectMicotoxinaspor
dc.subjectDigestão in vitropor
dc.subjectToxicologiapor
dc.subjectSegurança Alimentarpor
dc.subjectPatulinpor
dc.subjectIn vitro Digestionpor
dc.subjectCaco-2por
dc.subjectFruit Juicespor
dc.titleMycotoxins and applicability of in vitro methods of digestion and absorption assessment - the patulin case studypor
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0417%2F2012/PT
oaire.citation.conferencePlaceWageningen, Holandapor
oaire.citation.startPage83por
oaire.citation.title3rd Internatioal Conference on Food Digestion, 11-13 march 2014por
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor
relation.isProjectOfPublication7dee8a4a-7112-45ce-a8cb-8c552d7ae061
relation.isProjectOfPublication.latestForDiscovery7dee8a4a-7112-45ce-a8cb-8c552d7ae061

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