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Resistome, Virulome, and Clonal Variation in Methicillin-Resistant (MRSA) in Healthy Swine Populations: A Cross-Sectional Study

2024-04-24Journal article Open access
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorSilva, Adriana
dc.contributor.authorBarbero, Raquel
dc.contributor.authorRomero, Mario
dc.contributor.authorDel Campo, Rosa
dc.contributor.authorCaniça, Manuela
dc.contributor.authorCordeiro, Rui
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patricia
dc.date.accessioned2025-02-14T16:35:07Z
dc.date.available2025-02-14T16:35:07Z
dc.date.issued2024-04-24
dc.description(This article belongs to the Section Animal Genetics and Genomics)
dc.description.abstractThis cross-sectional study investigates the methicillin-resistant (MRSA): its prevalence, antimicrobial resistance, and molecular characteristics in healthy swine populations in central Portugal. A total of 213 samples were collected from pigs on twelve farms, and MRSA prevalence was assessed using selective agar plates and confirmed via molecular methods. Antimicrobial susceptibility testing and whole genome sequencing (WGS) were performed to characterize resistance profiles and genetic determinants. Among the 107 MRSA-positive samples (83.1% prevalence), fattening pigs and breeding sows exhibited notably high carriage rates. The genome of 20 isolates revealed the predominance of the ST398 clonal complex, with diverse types identified. Antimicrobial susceptibility testing demonstrated resistance to multiple antimicrobial agents, including penicillin, cefoxitin, and tetracycline. WGS analysis identified a diverse array of resistance genes, highlighting the genetic basis of antimicrobial resistance. Moreover, virulence gene profiling revealed the presence of genes associated with pathogenicity. These findings underscore the significant prevalence of MRSA in swine populations and emphasize the need for enhanced surveillance and control measures to mitigate zoonotic transmission risks. Implementation of prudent antimicrobial use practices and targeted intervention strategies is essential to reducing MRSA prevalence and safeguarding public health. Continued research efforts are warranted to elucidate transmission dynamics and virulence potential, ultimately ensuring food safety and public health protection.eng
dc.description.sponsorshipThis work received support and help from FCT/MCTES (LA/P/0008/2020 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2020, and UIDB/50006/2020 DOI 10.54499/UIDB/50006/2020).
dc.identifier.citationGenes (Basel). 2024 Apr 24;15(5):532. doi: 10.3390/genes15050532
dc.identifier.doi10.3390/genes15050532
dc.identifier.eissn2073-4425
dc.identifier.pmid38790161
dc.identifier.urihttp://hdl.handle.net/10400.18/10350
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationVeterinary and Animal Science Research Centre
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.hasversionhttps://www.mdpi.com/2073-4425/15/5/532
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMRSA
dc.subjectWGS
dc.subjectAntimicrobial Resistance
dc.subjectClonal Lineages
dc.subjectPigs
dc.subjectVirulence
dc.subjectResistência aos Antimicrobianos
dc.titleResistome, Virulome, and Clonal Variation in Methicillin-Resistant (MRSA) in Healthy Swine Populations: A Cross-Sectional Studyeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.citation.issue5
oaire.citation.startPage532
oaire.citation.titleGenes
oaire.citation.volume15
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublicatione683cb7c-d293-4738-83c6-c3c95d798e4d
relation.isProjectOfPublication72e49150-e3b3-4b5b-8e37-a5e3c312a767
relation.isProjectOfPublication226cfaf5-2eca-4b13-b46b-2f1c376c8172
relation.isProjectOfPublication.latestForDiscoverye683cb7c-d293-4738-83c6-c3c95d798e4d

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