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Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells: Correlation with polysomnographic and metabolic parameters

2016-11-15Journal article Open access
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dc.contributor.authorFeliciano, Amélia
dc.contributor.authorVaz, Fátima
dc.contributor.authorTorres, Vukosava M.
dc.contributor.authorValentim-Coelho, Cristina
dc.contributor.authorSilva, Rita
dc.contributor.authorProsinecki, Vesna
dc.contributor.authorAlexandre, Bruno M.
dc.contributor.authorCarvalho, Ana S.
dc.contributor.authorMatthiesen, Rune
dc.contributor.authorMalhotra, Atul
dc.contributor.authorPinto, Paula
dc.contributor.authorBárbara, Cristina
dc.contributor.authorPenque, Deborah
dc.date.accessioned2017-02-03T15:24:55Z
dc.date.available2017-11-16T01:30:12Z
dc.date.issued2016-11-15
dc.description.abstractWe have examined the effects of Obstructive Sleep Apnea (OSA) on red blood cell (RBC) proteome variation at evening/morning day time to uncover new insights into OSA-induced RBC dysfunction that may lead to OSA manifestations. Dysregulated proteins mainly fall in the group of catalytic enzymes, stress response and redox regulators such as peroxiredoxin 2 (PRDX2). Validation assays confirmed that at morning the monomeric/dimeric forms of PRDX2 were more overoxidized in OSA RBC compared to evening samples. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. Morning levels of overoxidized PRDX2 correlated with polysomnographic (PSG)-arousal index and metabolic parameters whereas the evening level of disulfide-linked dimer (associated with peroxidase activity of PRDX2) correlated with PSG parameters. After treatment, morning overoxidized multimer of PRDX2 negatively correlated with fasting glucose and dopamine levels. Overall, these data point toward severe oxidative stress and altered antioxidant homeostasis in OSA RBC occurring mainly at morning time but with consequences till evening. The beneficial effect of PAP involves modulation of the redox/oligomeric state of PRDX2, whose mechanism and associated chaperone/transduction signaling functions deserves further investigation. RBC PRDX2 is a promising candidate biomarker for OSA severity and treatment monitoring, warranting further investigation and validation.pt_PT
dc.description.sponsorshipProject partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics - Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), FCT/Poly-Annual Funding Program and FEDER/Saúde XXI Program (Portugal) and postdoctoral fellowship (SFRH/BPD/43365/2008) of Fundação para a Ciência e a Tecnologia (FCT), Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiochim Biophys Acta. 2017 Feb;1863(2):621-629. doi: 10.1016/j.bbadis.2016.11.019. Epub 2016 Nov 15pt_PT
dc.identifier.doi10.1016/j.bbadis.2016.11.019pt_PT
dc.identifier.issn0925-4439
dc.identifier.urihttp://hdl.handle.net/10400.18/4140
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925443916303015pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectSleep Apneiapt_PT
dc.subjectObstructive Sleep Apnept_PT
dc.subjectRed Blood Cellpt_PT
dc.subjectBiomarkerspt_PT
dc.subjectProteomicspt_PT
dc.subjectMetabolic Disorderspt_PT
dc.subjectGenomica Funcionalpt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleEvening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells: Correlation with polysomnographic and metabolic parameterspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.endPage629pt_PT
oaire.citation.startPage621pt_PT
oaire.citation.titleBBA - Biochimica et Biophysica Actapt_PT
oaire.citation.volume1863(2)pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica de copyright e de auto-arquivo do editor.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscoverye9cc9728-4f09-4e3a-b30d-53d4429986fb

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