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Genetic diversity and antifungal susceptibility patterns of Aspergillus nidulan complex obtained from clinical and environmental sources

2019-11-03Journal article Restricted access
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dc.contributor.authorTavakoli, Mahin
dc.contributor.authorRivero‐Menendez, Olga
dc.contributor.authorAbastabar, Mahdi
dc.contributor.authorHedayati, Mohammad T.
dc.contributor.authorSabino, Raquel
dc.contributor.authorSiopi, Maria
dc.contributor.authorZarrinfar, Hossein
dc.contributor.authorNouripour‐Sisakht, Sadegh
dc.contributor.authorLee, Henrich
dc.contributor.authorValadan, Reza
dc.contributor.authorMeletiadis, Joseph
dc.contributor.authorYazdani Charati, Jamshid
dc.contributor.authorSeyedmousavi, Seyedmojtaba
dc.contributor.authorAlastruey‐Izquierdo, Ana
dc.date.accessioned2020-04-24T11:49:57Z
dc.date.available2020-04-24T11:49:57Z
dc.date.issued2019-11-03
dc.description.abstractThe molecular epidemiology and antifungal susceptibility of Aspergillus nidulans species complex has not been well studied. To evaluate the genetic diversity and antifungal susceptibility patterns of clinical and environmental isolates of A. nidulans complex. Sixty clinical and environmental isolates of Aspergillus section Nidulantes were collected from five countries (Iran, The Netherlands, Spain, Portugal and Greece). The species were molecularly identified by sequencing of β-tubulin gene. The genetic diversity of A nidulans complex isolates (n = 54) was determined with a microsatellite genotyping assay. Antifungal susceptibility profile was determined using EUCAST method. The isolates were classified as A nidulans (46.7%), A spinulosporus (26.6%), A quadrilineatus (10%), A pachycristatus (3.3%), A rugulosus (3.3%), A unguis (5%), A creber, (1.7%), A olivicola (1.7%) and A sydowii (1.7%). Thirty-four sequence types (STs) were identified among the 54 A nidulans complex isolates. A high level of genetic diversity was found among A nidulans sensu stricto strains but low diversity was found among A spinulosporus strains. Amphotericin B showed high MICs to all species. The most active azole was posaconazole (GM = 0.64 mg/L), while itraconazole showed the highest MICs among azoles (GM = 2.95 mg/L). A spinulosporus showed higher MICs than A nidulans sensu stricto for all antifungals except for micafungin and anidulafungin. Interspecies variations may result in differences in antifungal susceptibility patterns and challenge antifungal therapy in infections caused by A nidulans. Differences in the distribution of STs or persistence of multiple STs might be related to the sources of isolation and niche specialisation.pt_PT
dc.description.sponsorshipThis project was supported in part by a research project from the Fondo de Investigación Sanitaria (PI16CIII/00035) and a research fund (No. 2819) from Invasive Fungi Research Center of Mazandaran University of Medical Sciences, Sari, Iran.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMycoses. 2020 Jan;63(1):78-88. doi: 10.1111/myc.13019. Epub 2019 Nov 3pt_PT
dc.identifier.doi10.1111/myc.13019pt_PT
dc.identifier.issn0933-7407
dc.identifier.urihttp://hdl.handle.net/10400.18/6510
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley/ Deutschsprachige Mykologische Gesellschaftpt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1111/myc.13019pt_PT
dc.subjectAspergillus nidulanspt_PT
dc.subjectMicrosatellitespt_PT
dc.subjectAspergilosispt_PT
dc.subjectAntifungal Susceptibilitypt_PT
dc.subjectβ-tubulinpt_PT
dc.subjectAspergillus spinulosporuspt_PT
dc.subjectAmphotericin Bpt_PT
dc.subjectCryptic Speciespt_PT
dc.subjectGenetic Diverspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleGenetic diversity and antifungal susceptibility patterns of Aspergillus nidulan complex obtained from clinical and environmental sourcespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage88pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage78pt_PT
oaire.citation.titleMycosespt_PT
oaire.citation.volume63pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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