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Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

2021-06-27Journal article Open access
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dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorLema-Arranz, Carlota
dc.contributor.authorRodríguez-Fernández, Raquel
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorReis, Ana Teresa
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta, Carla
dc.contributor.authorLaffon, Blanca
dc.date.accessioned2022-07-06T16:33:32Z
dc.date.available2022-07-06T16:33:32Z
dc.date.issued2021-06-27
dc.description(This article belongs to the Special Issue Cytotoxicity and Genotoxicity of Nanomaterials)pt_PT
dc.description.abstractMetal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO2), zinc oxide (ZnO), and cerium dioxide (CeO2) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use.pt_PT
dc.description.sponsorshipThis research was funded by Xunta de Galicia [ED431B 2019/02]; Ministerio de Ciencia e Innovación (PID2020-114908GA-I00); Ministerio de Educación, Cultura y Deporte [BEAGAL18/00142, to V.V.]; CA17140 NANO2CLINIC COST Action; NanoBioBarriers (PTDC/MED-TOX/31162/2017) funded by Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER/FNR) and through national funds by the Portuguese Foundation for Science and Technology (FCT). NanoLegaTox (POCI-01-0145-FEDER-029651) funded by Operational Program for Competitiveness and Internationalisation (COMPETE 2020) through FEDER, and through national funds by the Portuguese Foundation for Science and Technology (FCT). A.T. Reis funded by FCT Grant SFRH/BPD/122112/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomaterials (Basel). 2021 Jul 27;11(8):1930. doi: 10.3390/nano11081930pt_PT
dc.identifier.doi10.3390/nano11081930pt_PT
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10400.18/8078
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationA novelty study on the human uptake, genotoxicity and immunotoxicity of nanoparticles and legacy contaminants mixtures
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/11/8/1930pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCeO2 Nanoparticlespt_PT
dc.subjectTiO2 Nanoparticlespt_PT
dc.subjectZnO Nanoparticlespt_PT
dc.subjectComet Assaypt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectInterferencept_PT
dc.subjectSalivary Leukocytespt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleSalivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA novelty study on the human uptake, genotoxicity and immunotoxicity of nanoparticles and legacy contaminants mixtures
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F122112%2F2016/PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1930pt_PT
oaire.citation.titleNanomaterialspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream9471 - RIDTI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1370de00-d228-47a7-8aed-320831dc4dcd
relation.isProjectOfPublicationdbbc9b73-85f0-4bba-b64e-3624dc6b4d90
relation.isProjectOfPublication.latestForDiscovery1370de00-d228-47a7-8aed-320831dc4dcd

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