Publication
Translation of the human ABCE1 transcript is regulated by upstream open reading frames
| dc.contributor.author | Silva, Joana | |
| dc.contributor.author | Romão, Luísa | |
| dc.date.accessioned | 2020-05-21T18:34:42Z | |
| dc.date.embargo | 2025-12-31 | |
| dc.date.issued | 2019-06-11 | |
| dc.description.abstract | Short upstream open reading frames (uORFs) are cis-acting elements located within the 5'-leader sequence of transcripts and are defined by an initiation codon in-frame with a termination codon located upstream or downstream of its main ORF (mORF) initiation codon. Recent genome-wide ribosome profiling (Ribo-seq) studies have confirmed the widespread presence of uORFs and have shown that many uORFs can initiate with non-AUG codons. uORFs can impact gene expression of the downstream mORF by triggering mRNA decay or by regulating translation. Based on 5’-leader sequence ribosome occupancy profiles from Ribo-seq analysis in HCT116 cells, we studied the role of 6 non-AUG and 5 AUG uORFs present in the human ABCE1 mRNA. Using a set of reporter genes expressed in HCT116 cells and luminometry assays, we have observed that there are three AUG uORFs acting in a fail-safe manner to inhibit translation from the main AUG, being this repression immune to eIF2 phosphorylation. Functional aspects and implications of this regulatory mechanism to cell physiology will be discussed. | pt_PT |
| dc.description.sponsorship | Work partially funded by UID/MULTI/04046/2013 centre grant from FCT, Portugal (to BioISI), and by National Institute of Health Dr. Ricardo Jorge. J.F.P.S., is recipient of a fellowship from BioSys PhD programme (Ref SFRH/BD/106081/2015) from FCT (Portugal) | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6742 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.subject | Short Upstream Open Reading Frames | pt_PT |
| dc.subject | ABCE1 | pt_PT |
| dc.subject | Doenças Genéticas | pt_PT |
| dc.subject | Genómica Funcional e Estrutural | pt_PT |
| dc.title | Translation of the human ABCE1 transcript is regulated by upstream open reading frames | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Cracóvia, Polónia | pt_PT |
| oaire.citation.title | 24th Annual Meeting of the RNA Society, 11 June-16 June 2019 | pt_PT |
| rcaap.rights | embargoedAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- Poster Joana Silva e Luísa Romão_2019 .pdf
- Size:
- 1.72 MB
- Format:
- Adobe Portable Document Format
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: