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Assessment of multiple mycotoxins in maize flour samples from Portugal under SafeGrains project

2023-09Conference object Restricted access
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dc.contributor.authorDuarte, S.
dc.contributor.authorMagro, A.
dc.contributor.authorOliveira, C.
dc.contributor.authorRosim, R.
dc.contributor.authorAlvito, Paula
dc.contributor.authorCarvalho, M.O.
dc.date.accessioned2024-01-03T14:17:01Z
dc.date.available2024-01-03T14:17:01Z
dc.date.issued2023-09
dc.description.abstractOne of the most relevant problems in stored grains is the contamination by insects and mycotoxin-producing fungi. Tribolium castaneum is one of the most common insect pests of stored products. Its presence makes cereals more susceptible to the spread of the fungi Aspergillus flavus, which may produce mycotoxins. The aim of this work was to evaluate the mycotoxin profile of maize flour infested by T. castaneum adults, alone or in combination with mycotoxigenic A. flavus strain (MUM-UMinho). Maize collected directly from fields was stored at 4 ºC and then ground and sieved to obtain maize fl our. Multiple mycotoxin profile of maize flour was determined in three different assays: i) controls (n=8), ii) inoculated with T. castaneum adults (insect assay, n = 24), iii) inoculated with A. flavus conidia (fungus assay, n = 8) and iv) inoculated with both organisms (mixed assay, n = 24). Samples were analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) according to Franco et al. (2019) for determination of aflatoxins (AFs) B1, B2, G1 and G2, deoxynivalenol (DON), zearalenone(ZEN) and fumonisins (FBs) B1 and B2. The limits of detection (LOD) and quantification (LOQ) of the analytical method were 6.1 and 18.0 μg/ kg for DON, respectively. For AFs, ZEN and FBs, LOD and LOQ varied between 0.2 – 0.9 and 0.6 – 2.5 μg/kg, respectively. DON, ZEA, FB1 and FB2 were detected in control assays at mean levels ranging from 33-50, 2-6, 112-144 and 49-75 μg/kg, respectively. AFs were not detected in controls. Insect, fungi and mixed assays presented the same mycotoxin profile (with contents in the same order of magnitude), except for AFB1, which was detected only in mixed assays. Although the levels of mycotoxins are below the legislated ones (Commission Regulation (EU) 2023/915), they point out to the presence of multiple mycotoxins in raw maize flour and to a potential impact between insects and mycotoxigenic strains in which concerns AFB1. Data presented here agree with previous assays from this team describing significantly higher concentration in the maize flour inoculated with both organisms (Duarte et al, 2021).pt_PT
dc.description.sponsorshipThis study was supported by national funds from Portuguese Foundation for Science and Technology (FCT), through the research unit ID/AGR/04129/2013-LEAF and by the project SafeGrains (PTDC/ASP-PLA/28350/2017) and FCT/MEC – FEDER, within the PT2020 Partnership Agreement and Compete 2020. Thanks are also due to CESAM (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/2020), through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8824
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.subjectToxicologiapt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectSaúde Humanapt_PT
dc.subjectMicotoxinaspt_PT
dc.subjectPortugalpt_PT
dc.titleAssessment of multiple mycotoxins in maize flour samples from Portugal under SafeGrains projectpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FASP-PLA%2F28350%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0094%2F2020/PT
oaire.citation.conferencePlaceCampinas, Brasilpt_PT
oaire.citation.title5th International Conference on Food Contaminants (ICFC2023), 4-6 September 2023pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isProjectOfPublicationcc47ebcf-cbf8-4958-858e-49b0d2690634
relation.isProjectOfPublication4e22de31-8fc5-4b15-b980-3fd0edf5515d
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relation.isProjectOfPublicationbc5e46b5-0da4-4ee2-97e0-bf2ab95a5870
relation.isProjectOfPublication.latestForDiscovery0e72e6c8-b610-43e0-931c-8ad275ad6093

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