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Microbial gut evaluation in an angolan paediatric population with sickle cell disease

2022-11Journal article Open access
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dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorGinete, Catarina
dc.contributor.authorSantos, Brígida
dc.contributor.authorMendes, Joana
dc.contributor.authorMiranda, Armandina
dc.contributor.authorVasconcelos, Jocelyne
dc.contributor.authorBrito, Miguel
dc.date.accessioned2023-02-08T15:53:50Z
dc.date.available2023-02-08T15:53:50Z
dc.date.issued2022-11
dc.description.abstractSickle cell disease (SCD) is one of the most common genetic conditions worldwide. It can contribute up to 90% of under-5 mortality in sub-Saharan Africa. Clinical manifestations are very heterogeneous, and the intestinal microbiome appears to be crucial in the modulation of inflammation, cell adhesion and induction of aged neutrophils, the main interveners of recurrent vaso-occlusive crisis. Enterocyte injury, increased permeability, altered microbial composition and bacterial overgrowth have all been documented as microbial and pathophysiologic changes in the gut microbiome of SCD patients in recent studies. Our aim was to sequence the bacterial 16S rRNA gene in order to characterize the gut microbiome of Angolan children with SCA and healthy siblings as a control. A total of 72 stool samples were obtained from children between 3 and 14 years old. Our data showed that the two groups exhibit some notable differences in microbiota relative abundance at different classification levels. Children with SCA have a higher number of the phylum Actinobacteria. As for the genus level, Clostridium cluster XI bacteria was more prevalent in the SCA children, whereas the siblings had a higher abundance of Blautia, Aestuariispira, Campylobacter, Helicobacter, Polaribacter and Anaerorhabdus. In this study, we have presented the first microbiota analysis in an Angolan paediatric population with SCD and provided a detailed view of the microbial differences between patients and healthy controls. There is still much to learn before fully relying on the therapeutic approaches for gut modulation, which is why more research in this field is crucial to making this a reality.pt_PT
dc.description.sponsorshipThis project was supported by FCT/Aga Khan (project n°330842553), FCT/MCTES (UIDB/05608/2020 and UIDP/05608/2020) –H&TRC. The authors would like to acknowledge FCT for the financial sup port of the fellowships for M.D. (UI/BD/150705/2020) and C.G. (330842553/SCAFfoldChild/2020/BI/12 M)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Cell Mol Med. 2022 Nov;26(21):5360-5368. doi: 10.1111/jcmm.17402. Epub 2022 Sep 28.pt_PT
dc.identifier.doi10.1111/jcmm.17402pt_PT
dc.identifier.issn1582-1838
dc.identifier.urihttp://hdl.handle.net/10400.18/8516
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationHealth and Technology Research Center
dc.relationHealth and Technology Research Center
dc.relationGut Microbiome Analysis in Patients with Sickle Cell Disease
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/jcmm.17402pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subject16S rRNApt_PT
dc.subjectFoetal Haemoglobinpt_PT
dc.subjectMicrobiomept_PT
dc.subjectSickle Cell Diseasept_PT
dc.subjectAngolapt_PT
dc.subjectPatologias do Glóbulo Vermelhopt_PT
dc.titleMicrobial gut evaluation in an angolan paediatric population with sickle cell diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleHealth and Technology Research Center
oaire.awardTitleGut Microbiome Analysis in Patients with Sickle Cell Disease
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F05608%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/UI%2FBD%2F150705%2F2020/PT
oaire.citation.endPage5368pt_PT
oaire.citation.issue21pt_PT
oaire.citation.startPage5360pt_PT
oaire.citation.titleJournal of Cellular and Molecular Medicinept_PT
oaire.citation.volume26pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication875a9c35-558d-4af9-abf0-254eadf9819d
relation.isProjectOfPublicationc9a7f44a-b944-4ea0-a930-85b8e0b1bf1d
relation.isProjectOfPublication1317b5f3-f77d-4e28-9a47-40d11d6fc905
relation.isProjectOfPublication.latestForDiscovery875a9c35-558d-4af9-abf0-254eadf9819d

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