Publication
Imported poultry meat as a source of extended-spectrum cephalosporin-resistant CMY-2-producing Salmonella Heidelberg and Salmonella Minnesota in the European Union, 2014-2015
| dc.contributor.author | Campos, Joana | |
| dc.contributor.author | Mourão, Joana | |
| dc.contributor.author | Silveira, Leonor | |
| dc.contributor.author | Saraiva, Margarida | |
| dc.contributor.author | Correia, Cristina Belo | |
| dc.contributor.author | Maçãs, Ana Paula | |
| dc.contributor.author | Peixe, Luísa | |
| dc.contributor.author | Antunes, Patrícia | |
| dc.date.accessioned | 2018-03-22T17:57:37Z | |
| dc.date.available | 2018-03-22T17:57:37Z | |
| dc.date.issued | 2017-09-14 | |
| dc.description.abstract | Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of poultry meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from poultry meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations. | pt_PT |
| dc.description.sponsorship | This work was supported by FCT/MEC (Fundação para a Ciência e a Tecnologia/Ministério da Educação e Ciência) through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 [grant no. UID/MULTI/04378/2013 – POCI/01/ 0145/FERDER/007728]; the project NORTE-01-0145-FEDER -000011 – Qualidade e Segurança Alimentar—uma abordagem (nano)tecnológica. JC is supported by a PhD fellowship from FCT [grant no. SFRH/BD/93091/2013] and JM is supported by a postdoctoral fellowship from project NORTE-01-0145-FEDER-000024. The authors are greatly indebted to all of the funding sources. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Int J Antimicrob Agents. 2018 Jan;51(1):151-154. doi: 10.1016/j.ijantimicag.2017.09.006. Epub 2017 Sep 14. | pt_PT |
| dc.identifier.doi | 10.1016/j.ijantimicag.2017.09.006 | pt_PT |
| dc.identifier.issn | 0924-8579 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/5443 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Elsevier/International Society of Chemotherapy | pt_PT |
| dc.relation.publisherversion | http://www.ijaaonline.com/article/S0924-8579(17)30346-1/fulltext | pt_PT |
| dc.subject | Extended-spectrum Cephalosporin Resistance | pt_PT |
| dc.subject | Imported Poultry Meat | pt_PT |
| dc.subject | Salmonella Heidelberg | pt_PT |
| dc.subject | Salmonella Minnesota | pt_PT |
| dc.subject | Salmonella enterica | pt_PT |
| dc.subject | bla(CMY-2) | pt_PT |
| dc.subject | Infecções Gastrointestinais | pt_PT |
| dc.subject | Segurança Alimentar | pt_PT |
| dc.title | Imported poultry meat as a source of extended-spectrum cephalosporin-resistant CMY-2-producing Salmonella Heidelberg and Salmonella Minnesota in the European Union, 2014-2015 | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 154 | pt_PT |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 151 | pt_PT |
| oaire.citation.title | International Journal of Antimicrobial Agents | pt_PT |
| oaire.citation.volume | 51 | pt_PT |
| rcaap.rights | embargoedAccess | pt_PT |
| rcaap.type | article | pt_PT |