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Neurotoxicity assessment of oleic acid-coated iron oxide nanoparticles in SH-SY5Y cells

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dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorCosta, Carla
dc.contributor.authorBrandão, Fátima
dc.contributor.authorKiliç, Gözde
dc.contributor.authorTeixeira, Joao Paulo
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.date.accessioned2019-03-25T17:10:52Z
dc.date.available2019-03-25T17:10:52Z
dc.date.issued2018
dc.description.abstractIron oxide nanoparticles (ION) awaken a particular interest for biomedical applications due to their unique physicochemical properties, especially superparamagnetism, and ability to cross the blood-brain barrier. ION surface can be coated to improve their properties and facilitate functionalization. Still, coating may affect toxicity. The aim of this work was to evaluate the possible effects of oleic acid-coated ION (O-ION) on human neuronal cells (SH-SY5Y). A set of assays was conducted in complete and serum-free culture media for 3 and 24 h to assess O-ION cytotoxic effects - cell membrane disruption, cell cycle alteration and cell death induction -, and genotoxic effects - primary DNA damage, H2AX phosphorylation and micronuclei induction -, considering also DNA repair competence and iron ion release. Results obtained show that O-ION exhibit a moderate cytotoxicity related to cell membrane impairment, cell cycle disruption and cell death induction, especially notable in serum-free medium. Iron ion release was only observed in complete medium, indicating that cytotoxicity observed was not related to the presence of ions in the medium. However, O-ION genotoxic effects were limited to the induction of primary DNA damage, not related to double strand breaks, and this damage did not become fixed in cells in most conditions. Alterations in repair ability (DNA repair competence assay) were observed when cells where treated with O-ION before or during the challenge with H2O2, but not during the repair period. Further investigation is needed to clarify the possible role of oxidative stress and protein corona on observed O-ION toxicity.pt_PT
dc.description.sponsorshipThis work was funded by Xunta de Galicia (ED431B 2016/013). V. Valdiglesias was supported by a Xunta de Galicia postdoctoral fellowship (reference ED481B 2016/190-0). N. Fernández-Bertólez was supported by an INDITEX-UDC fellowship, and Fatima Brandão by the Porthuguese Foundation for Science and Technology (SFRH/ BD101060/2014).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationToxicology. 2018 Aug 1;406-407:81-91. doi: 10.1016/j.tox.2018.06.003. Epub 2018 Jun 6.pt_PT
dc.identifier.doi10.1016/j.tox.2018.06.003pt_PT
dc.identifier.issn0300-483X
dc.identifier.urihttp://hdl.handle.net/10400.18/6303
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ British Toxicology Societypt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0300483X18301112?via%3Dihubpt_PT
dc.subjectCell Cyclept_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectCell Survivalpt_PT
dc.subjectDose-Response Relationship, Drugpt_PT
dc.subjectFerric Compoundspt_PT
dc.subjectHumanspt_PT
dc.subjectMetal Nanoparticlespt_PT
dc.subjectOleic Acidpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectIron Oxide Nanoparticlespt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectSH-SY5Y Cellspt_PT
dc.subjectNeurotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleNeurotoxicity assessment of oleic acid-coated iron oxide nanoparticles in SH-SY5Y cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage91pt_PT
oaire.citation.startPage81pt_PT
oaire.citation.titleToxicologypt_PT
oaire.citation.volume406-407pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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