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Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance

2022-05Journal article Open access
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dc.contributor.authorPinto, Miguel
dc.contributor.authorBorges, Vítor
dc.contributor.authorNascimento, Maria
dc.contributor.authorMartins, Filomena
dc.contributor.authorPessanha, Maria Ana
dc.contributor.authorFaria, Isabel
dc.contributor.authorRodrigues, João
dc.contributor.authorMatias, Rui
dc.contributor.authorGomes, João Paulo
dc.contributor.authorJordao, Luisa
dc.date.accessioned2022-11-18T14:49:23Z
dc.date.available2024-01-01T01:30:56Z
dc.date.issued2022-05
dc.descriptionObservational Study
dc.description.abstractBackground: Central venous catheter-related bloodstream infection (CRBSI) is a huge public health concern with considerable impact on mortality and health costs. Aim: A three-year observational study enrolling three tertiary hospitals located in Lisbon, Portugal, was designed to identify the major aetiological agents of CRBSI, their ability to colonize central venous catheters and their antimicrobial resistance profiles. Methods: Aetiological agents of CRBSI were identified by Vitek 2. Whole-genome sequencing was used to confirm CRBSI by the most prevalent aetiological agents and characterize their resistome. Central venous catheter colonization (namely by biofilm assembly) was monitored by scanning electron microscopy. Findings: Staphylococci were the most prevalent causative agent (36/58, 62.0%), with S. aureus and coagulase-negative S. epidermidis accounting for 24.1% and 36.2% of CRBSIs, respectively. Fifty-nine of 72 staphylococci isolates were meticillin resistant. Comparative genomic analysis of central venous catheters/haemoculture pairs of isolates revealed genomic matches for 35 of 36 pairs and a good correlation between antibiotic susceptibility phenotype and the presence of antimicrobial resistance genetic determinants. Biofilms were present on 48.6% of the central venous catheters; nevertheless, no statistically significant association was established between biofilm assembly and CRBSI, and the presence/absence of ica operon and agr groups did not correlate with biofilm phenotypes, highlighting the need for further studies to elucidate biofilms' role on this healthcare-associated infection. Conclusion: Whole-genome sequencing was shown to be a valuable tool to confirm CRBSI. Although more than 42.3% of the central venous catheters were colonized by staphylococci, no statistically significant association was found between CRBSI and biofilms.pt_PT
dc.description.sponsorshipThis research was partially funded by Fundação para a Ciência e a Tecnologia (FCT) as part of the Bilateral Cooperation Program between Portugal and Slovakia 2019-2021 (Grant FCT/487/15/01/2019/S).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Hosp Infect. 2022 May;123:43-51. doi: 10.1016/j.jhin.2022.01.025. Epub 2022 Feb 18pt_PT
dc.identifier.doi10.1016/j.jhin.2022.01.025pt_PT
dc.identifier.issn0195-6701
dc.identifier.urihttp://hdl.handle.net/10400.18/8321
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/The Healthcare Infection Societypt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0195670122000573?via%3Dihubpt_PT
dc.subjectCatheter-related Bloodstream Infectionpt_PT
dc.subjectCatheter-Related Infectionspt_PT
dc.subjectStaphylococcus Spidermidispt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectBiofilmspt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectWhole-genome Sequencingpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleInsights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage51pt_PT
oaire.citation.startPage43pt_PT
oaire.citation.titleJournal of Hospital Infectionpt_PT
oaire.citation.volume123pt_PT
rcaap.embargofctDe acordo com a política da editorapt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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