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Titanium Dioxide Nanoparticles molecular effects: Internalization in the Human Intestinal Epithelium

2021-09-23Documento de conferência Acesso restrito
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dc.contributor.authorRolo, Dora
dc.contributor.authorPereira, Joana FS
dc.contributor.authorMatos, Paulo
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorBettencourt, Ana Francisca
dc.contributor.authorJordan, Peter
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-01-13T17:53:18Z
dc.date.available2022-01-13T17:53:18Z
dc.date.issued2021-09-23
dc.descriptionAbstract publicado em: Environ Mol Mutagen. 2021;62(S1):42 (P11). https://onlinelibrary.wiley.com/doi/epdf/10.1002/em.22458
dc.description.abstractThe use of titanium dioxide nanoparticles (TiO2-NPs) as food additive and in food packaging demands a thorough assessment of their potential risk for human health, specifically with regard to gastrointestinal tract. Using intestinal cells, we analyzed the mechanisms by which digested TiO2-NPs (NM-102, NM-103 and NM-105, JRC repository) translocate through the intestinal epithelium layer, as compared to non-digested particles. Human digestion of TiO2-NPs was simulated using the INFOGEST in vitro harmonized digestion method. Caco-2 cells were grown as polarized cell monolayer for exposure and differentiation was evaluated by TEER. The translocation of TiO2-NPs, tagged with alizarin red, through the cell barrier was analysed by confocal microscopy, using colocalization with antibodies against specific endosomal compartments. The internalization of the TiO2-NPs was confirmed for the three TiO2-NPs tested, both before and after digestion simulation. The smallest TiO2-NPs were internalized into EEA1-positive early- endosomes and accumulated in late endosomes (Rab7), with only a small fraction following the degradative pathway to the lysosome (LAMP1). The data suggested that at least part of the TiO2-NPs could be redirected to the secretory pathway. Consistently, fluorescence passing from the apical to the basolateral chamber was observed, without disruption of the intestinal barrier function. The changes in cell function or signal transduction pathways are being studied and possible consequences to human gastrointestinal tract arediscussed. We thank the support from P. Alvito, C. Martins and R. Assunção (INSA,Lisbon, Portugal).pt_PT
dc.description.sponsorshipFunded by FCT/MCTES-PIDDAC,PTDC/SAU-PUB/29481/2017, iMed.ULisboa (UID/DTP/04138/2019; UIDB/04138/2020); ToxOmics(UIDB/00009/2020; UIDP/00009/2020) and CEECIND/03143/2017 (L. Gonçalves).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7849
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCEECIND/03143/2017pt_PT
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectTtitanium Dioxidept_PT
dc.subjectNanomaterialspt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectIntestinal Cellspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectVias de sinalização e patologias associadaspt_PT
dc.titleTitanium Dioxide Nanoparticles molecular effects: Internalization in the Human Intestinal Epitheliumpt_PT
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dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlace(online)pt_PT
oaire.citation.title52nd Annual Meeting Environmental Mutagenesis & Genomics Society (EMGS), 22-25 September 2021 (online)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
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person.familyNameSilva
person.familyNameLouro
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
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