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Characterization through multilocus sequence analysis of Borrelia turdi isolates from Portugal

2015-08-27Journal article Restricted access
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dc.contributor.authorNorte, Ana Cláudia
dc.contributor.authorAraújo, Pedro Miguel
dc.contributor.authorPascoal da Silva, Luís
dc.contributor.authorTenreiro, Paulo Quadros
dc.contributor.authorRamos, Jaime A
dc.contributor.authorNúncio, Maria Sofia
dc.contributor.authorZé-Zé, Líbia
dc.contributor.authorLopes de Carvalho, Isabel
dc.date.accessioned2016-02-18T15:11:06Z
dc.date.available2016-02-18T15:11:06Z
dc.date.issued2015-08-27
dc.description.abstractBorrelia turdi is a spirochete from the Borrelia burgdorferi complex, first reported in Japan, that has been increasingly detected in Europe. This genospecies is mostly associated with avian hosts and their ornithophilic ticks such as Ixodes frontalis. In this study, we isolated B. turdi from five I. frontalis feeding on Turdus merula, Turdus philomelos, Parus major and Troglodytes troglodytes, and one Ixodes ricinus feeding on a T. merula in Portugal. These isolates were genetically characterised according to their 5S-23S rRNA intergenic spacer, 16S rRNA and through typing of seven housekeeping genes (multilocus sequence typing). Multilocus sequence analyses revealed that the strains isolated in our study, although belonging to B. turdi genospecies, are not identical to the B. turdi reference strain Ya501. Instead, our strains are separated into a clear defined group, suggesting that the European samples diverged genetically from the strain originally detected in Japan. Population analysis of 5S-23S rRNA sequences can further resolve subpopulations within B. turdi, but more samples from a large geographical scale and host range would be needed to assess potential phylogeographical patterns within this genospecies.pt_PT
dc.description.sponsorshipAna C. Norte was financially supported by a post-doctoral fellowship from the Portuguese Foundation for Science and Technology (SFRH/BPD/62898/2009), and the study was partially funded by a centre grant (to BioISI, Centre Reference: UID/MULTI/04046/2013) from FCT/MCTES/PIDDAC, Portugal.
dc.identifier.citationMicrob Ecol. 2016 Nov;72(4):831-839. doi:10.1007/s00248-015-0660-1. Epub 2015 Aug 27pt_PT
dc.identifier.doi10.1007/s00248-015-0660-1pt_PT
dc.identifier.issn0095-3628
dc.identifier.urihttp://hdl.handle.net/10400.18/3409
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Science, Business Media New Yorkpt_PT
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00248-015-0660-1pt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.subjectBorrelia turdipt_PT
dc.subjectMultilocus Sequence Typingpt_PT
dc.subjectIsolatept_PT
dc.subjectPortugalpt_PT
dc.subjectIxodes Frontalispt_PT
dc.subjectPhylogenetic Analysispt_PT
dc.titleCharacterization through multilocus sequence analysis of Borrelia turdi isolates from Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04046%2F2013/PT
oaire.citation.titleMicrobial Ecologypt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationdc84f768-e6f2-4eea-b294-6c8ebbd1a156
relation.isProjectOfPublication.latestForDiscoverydc84f768-e6f2-4eea-b294-6c8ebbd1a156

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