Publication
First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains
| dc.contributor.author | de Beer, Jessica | |
| dc.contributor.author | Kremer, Kristin | |
| dc.contributor.author | Ködmön, Csaba | |
| dc.contributor.author | Supply, Philip | |
| dc.contributor.author | van Soolingen, Dick | |
| dc.contributor.author | Global Network for the Molecular Surveillance of Tuberculosis 2009 | |
| dc.date.accessioned | 2012-10-25T11:34:15Z | |
| dc.date.available | 2012-10-25T11:34:15Z | |
| dc.date.issued | 2011-12-14 | |
| dc.description.abstract | Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future. | por |
| dc.description.sponsorship | Institute for Public Health and the Environment (RIVM), European Centre for Diseases Prevention and Control (ECDC) | por |
| dc.identifier.citation | J Clin Microbiol. 2012 Mar;50(3):662-9. Epub 2011 Dec 14 | por |
| dc.identifier.issn | 0095-1137 | |
| dc.identifier.other | doi:10.1128/JCM.00607-11 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1071 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | American Society for Microbiology | por |
| dc.relation | Molecular Surveillance of Multidrug Resistant Tuberculosis in Europe | por |
| dc.relation.publisherversion | http://jcm.asm.org/content/50/3/662 | por |
| dc.subject | Variable Number Tandem Repeat (VNTR) | por |
| dc.subject | Multiple-Locus Variable number tandem repeat Analysis (MLVA) | por |
| dc.subject | Tuberculosis | por |
| dc.subject | proficiency | por |
| dc.subject | Quality Control | por |
| dc.subject | Reproducibility | por |
| dc.subject | Tandem Repeats | por |
| dc.subject | Resistência aos Antimicrobianos | por |
| dc.title | First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 669 | por |
| oaire.citation.startPage | 662 | por |
| oaire.citation.title | Journal of Clinical Microbiology | por |
| oaire.citation.volume | 50 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |