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Genotyping of Coxiella burnetii in sheep and goat abortion samples

2018-12-04Journal article Open access
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dc.contributor.authorChochlakis, Dimosthenis
dc.contributor.authorSantos, Ana Sofia
dc.contributor.authorGiadinis, Nektarios D.
dc.contributor.authorPapadopoulos, Dimitrios
dc.contributor.authorBoubaris, Leonidas
dc.contributor.authorKalaitzakis, Emmanouil
dc.contributor.authorPsaroulaki, Anna
dc.contributor.authorKritas, Spyridon K.
dc.contributor.authorPetridou, Evanthia I.
dc.date.accessioned2019-03-20T15:17:30Z
dc.date.available2019-03-20T15:17:30Z
dc.date.issued2018-12-04
dc.description.abstractBackground: Q fever, caused by Coxiella burnetii, is a zoonosis that presents a worldwide distribution and affects both humans and animals. The route of dispersal of the pathogen by ruminants into the environment usually involves stages of abortion and parturition, nevertheless the agent can, also, be detected in other animal samples. Therefore it is considered as important in terms of proper diagnosis, as well as, for epidemiology and surveillance purposes, to genotype the pathogen. The aim of the current study was to investigate the presence of different genotypes of the agent in animals that had suffered from abortion during a two-year survey in Greece. Results: Sixty nine tissue samples (37 stomach contents, 11 liver samples, 21 cotyledons) were collected from 59 abortion cases in sheep (N = 45) and goats (N = 14) from 65 farms at eight different areas of Greece. Samples were screened by qPCR and positive ones were further genotyped using a 10-locus multiple loci (ms 1, 3, 7, 12, 20, 21, 22, 26, 30 and 36) variable number of tandem repeat analysis (MLVA) method. Three genotypes were identified in sheep (A, B, C). Samples representing each of the obtained MLVA profile were further used for MST genotyping. Ten spacers (Cox 2, 5, 6, 18, 20, 22, 37, 51, 56 and 57) were amplified. A close relatedness among the identified MLVA genotypes was confirmed since they all belonged to MST group 32. Conclusions: The current study introduces into the aspect of genotyping of C. burnetii in Greece. Further studies are needed to explore the presence of more genotypes, to associate the genotypes circulating in the animal and tick population with those causing human disease in order to further expand on the epidemiological aspects of the pathogen.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBMC Microbiol. 2018 Dec 4;18(1):204. doi: 10.1186/s12866-018-1353-y.pt_PT
dc.identifier.doi10.1186/s12866-018-1353-ypt_PT
dc.identifier.issn1471-2180
dc.identifier.urihttp://hdl.handle.net/10400.18/6242
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMC (part of Springer Nature)pt_PT
dc.relation.publisherversionhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-018-1353-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAbortionpt_PT
dc.subjectCoxiella burnetiipt_PT
dc.subjectMLVApt_PT
dc.subjectMSTpt_PT
dc.subjectSmall Ruminantspt_PT
dc.subjectQ feverpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleGenotyping of Coxiella burnetii in sheep and goat abortion samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage204pt_PT
oaire.citation.titleBMC Microbiologypt_PT
oaire.citation.volume18pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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