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Campylobacter jejuni in Different Canine Populations: Characteristics and Zoonotic Potential

2021-10-26Journal article Open access
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dc.contributor.authorLemos, Maria-Leonor
dc.contributor.authorNunes, Alexandra
dc.contributor.authorAncora, Massimo
dc.contributor.authorCammà, Cesare
dc.contributor.authorCosta, Paulo Martins da
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2022-07-03T15:49:45Z
dc.date.available2022-07-03T15:49:45Z
dc.date.issued2021-10-26
dc.description.abstractWith most epidemiological studies focused on poultry, dogs are often overlooked as a reservoir of Campylobacter, even though these animals maintain close daily contact with humans. The present study aimed to obtain a first insight into the presence and characteristics of Campylobacter spp. in different canine populations in Portugal, and to evaluate its zoonotic potential through genomic analysis. From a total of 125 rectal swabs collected from companion (n = 71) and hunting dogs (n = 54) living in two different settings, rural (n = 75) and urban (n = 50), 32 Campylobacter spp. isolates were obtained. Four different Campylobacter species were identified by Multiplex PCR and MALDI-TOF mass spectrometry, of which Campylobacter jejuni (n = 14, 44%) was overall the most frequently found species. Relevant resistance phenotypes were detected in C. jejuni, with 93% of the isolates being resistant to ciprofloxacin, 64% to tetracycline, and 57% to ampicillin, and three isolates being multi-drug-resistant. Comparison of the phenotypic and genotypic traits with human isolates from Portuguese patients revealed great similarity between both groups. Particularly relevant, the wgMLST analysis allowed the identification of isolates from human and dogs without any apparent epidemiological relationship, sharing high genetic proximity. Notwithstanding the limited sample size, considering the high genomic diversity of C. jejuni, the genetic overlap between human and dog strains observed in this study confirmed that the occurrence of this species in dogs is of public health concern, reinforcing the call for a One Health approach.pt_PT
dc.description.sponsorshipThis research was partially funded by the Italian Ministry of Health, grant No IZS AM 02/19 RC.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2021 Oct 26;9(11):2231. doi: 10.3390/microorganisms9112231pt_PT
dc.identifier.doi10.3390/microorganisms9112231pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/8049
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/9/11/2231pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCampylobacter spp.pt_PT
dc.subjectOne Healthpt_PT
dc.subjectDogspt_PT
dc.subjectWhole-Genome Sequencingpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleCampylobacter jejuni in Different Canine Populations: Characteristics and Zoonotic Potentialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11pt_PT
oaire.citation.startPage2231pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume9pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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