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The impact of comet assay data normalization in human biomonitoring studies outcomes

2020-07-01Artigo científico Acesso restrito
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dc.contributor.authorEsteves, Filipa
dc.contributor.authorAmaro, Raquel
dc.contributor.authorSilva, Susana
dc.contributor.authorSánchez-Flores, María
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta, Carla
dc.date.accessioned2020-10-27T19:09:00Z
dc.date.available2020-10-27T19:09:00Z
dc.date.issued2020-07-01
dc.description.abstractThe comet assay has been extensively used in biomonitoring studies. To avoid intra-experimental variability, the incorporation of assay controls in each work session for data normalization has been suggested by some authors but has never been thoroughly analyzed. The aim of this study was to address the impact of data normalization in the results of a biomonitoring study using different normalization models. Human peripheral blood mononuclear cells (PBMC) from 140 healthy individuals were analyzed using the alkaline and FPG-modified version of the comet assay across seven different work sessions. In addition to negative standards, methyl methanesulfonate (MMS) and Ro 19−8022 plus light treated PBMC, were also included in the assay as positive standards. To verify the impact of data normalization, some demographic, lifestyle and environmental exposure-related variables were selected. Significant associations with independent study variables were observed using normalized comet endpoints, as opposed to raw data. After normalization, levels of DNA strand breaks were significantly higher among males and older individuals (>71 years), while net FPG-sensitive sites were positively related to smoking habits and environmental exposures (i.e. air pollution and bottled water consumption). This study highlights how the normalization strategies can influence the statistical results of a human biomonitoring study and lead to different data interpretations.pt_PT
dc.description.sponsorshipThis work was supported by Project NORTE-01-0145-FEDER000010 - Health, Comfort and Energy in the Built Environment (HEBE), cofinanced by Programa Operacional Regional do Norte (NORTE2020), through Fundo Europeu de Desenvolvimento Regional (FEDER). Carla Costa is supported by the FCT post-doctoral grant SFRH/BPD/96196/2013. The authors would also like to acknowledge the contribution of the hCOMET CA15132 COST Action.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationToxicol Lett. 2020 Oct 10;332:56-64. doi: 10.1016/j.toxlet.2020.06.024. Epub 2020 Jul 1pt_PT
dc.identifier.doi10.1016/j.toxlet.2020.06.024pt_PT
dc.identifier.issn0378-4274
dc.identifier.urihttp://hdl.handle.net/10400.18/7223
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ EUROTOXpt_PT
dc.relationImpact of transplacental exposure to tobacco smoke in the DNA of newborn. Evaluation of genetic damage and epigenetic changes. Evolution throughout childhood.
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0378427420303027?via%3Dihubpt_PT
dc.subjectData Normalizationpt_PT
dc.subjectAssay Controlspt_PT
dc.subjectComet Assaypt_PT
dc.subjectHuman Biomonitoringpt_PT
dc.subjectFPGpt_PT
dc.subjectToxicologiapt_PT
dc.titleThe impact of comet assay data normalization in human biomonitoring studies outcomespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImpact of transplacental exposure to tobacco smoke in the DNA of newborn. Evaluation of genetic damage and epigenetic changes. Evolution throughout childhood.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F96196%2F2013/PT
oaire.citation.endPage64pt_PT
oaire.citation.startPage56pt_PT
oaire.citation.titleToxicology Letterspt_PT
oaire.citation.volume332pt_PT
person.familyNameEsteves
person.givenNameFilipa
person.identifierAAS-7291-2020
person.identifier.ciencia-id9C18-72CD-9F47
person.identifier.orcid0000-0001-9187-0279
person.identifier.scopus-author-id57194834020
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationee07413d-0682-42cf-a7aa-b3115d710b9f
relation.isAuthorOfPublication.latestForDiscoveryee07413d-0682-42cf-a7aa-b3115d710b9f
relation.isProjectOfPublicationf4b22fdf-07e9-4ee8-884a-ea80e97bc850
relation.isProjectOfPublication.latestForDiscoveryf4b22fdf-07e9-4ee8-884a-ea80e97bc850

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