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The Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activities

2022-06-30Journal article Open access
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dc.contributor.authorFaleiro, Leonor
dc.contributor.authorMarques, Ana
dc.contributor.authorMartins, João
dc.contributor.authorJordão, Luísa
dc.contributor.authorNogueira, Isabel
dc.contributor.authorGumerova, Nadiia I.
dc.contributor.authorRompel, Annette
dc.contributor.authorAureliano, Manuel
dc.date.accessioned2023-01-16T14:31:59Z
dc.date.available2023-01-16T14:31:59Z
dc.date.issued2022-06-30
dc.descriptionThis article belongs to the Special Issue Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions.pt_PT
dc.description.abstractSimple Summary: Besides showing the antibacterial activity of the Preyssler-type polyoxotungstate (POT) P5W30 with the ability to affect MRSA cells, we demonstrated that P5W30 also displays other proprieties, such as anti-quorum sensing and antibiofilm. These are biological activities that are reported for a POT for the first time. Quorum sensing and biofilm facilitate the bacterial colonization, antibiotic resistance and persistence in both the environment and host, and its impairment by POTs can greatly contribute to the control of bacterial infections, such as those caused by multiresistant bacteria. Moreover, antiviral activity was also observed using the enterovirus Qβ. NMR stability studies of P5W30 demonstrate that it remains intact, suggesting its responsibility in the described biological activities. Taken together, our results emphasize the potential biomedical use of POTs, particularly the Preyssler-type POT, to fight antibiotic-resistant MRSA strains and their ability to form biofilm, besides being a promising antiviral agent.pt_PT
dc.description.abstractAbstract: The increase in bacterial resistance to antibiotics has led researchers to find new compounds or find combinations between different compounds with potential antibacterial action and with the ability to prevent the development of antibiotic resistance. Polyoxotungstates (POTs) are inorganic clusters that may fulfill that need, either individually or in combination with antibiotics. Herein, we report the ability of the polyoxotungstates (POTs) with Wells-Dawson P2W18, P2W17, P2W15, and Preyssler P5W30 type structures to differently affect Gram-negative and Gram-positive microorganisms, either susceptible or resistant to antibiotics. The compound P5W30 showed the highest activity against the majority of the tested bacterial strains in comparison with the other tested POTs (P2W15, P2W17 and P2W18) that did not show inhibition zones for the Gram-negative bacteria, A. baumanii I73775, E. coli DSM 1077, E. coli I73194, K. pneumoniae I7092374, and P. aeruginosa C46281). Generally, the results evidenced that Gram-positive bacteria are more susceptible to the POTs tested. The compound P5W30 was the one most active against S. aureus ATCC 6538 and MRSA16, reaching <0.83 mg·mL−1 (100 μM) and 4.96 mg·mL−1 (600 μM), respectively. Moreover, it was verified by NMR spectroscopy that the most promising POT, P5W30, remains intact under all the experimental conditions, after 24 h at 37 °C. This prompted us to further evaluate the anti-quorum sensing activity of P5W30 using the biosensor Chromobacterium violaceum CV026, as well as its antibiofilm activity both individually and in combination with the antibiotic cefoxitin against the methicillin-resistant Staphylococcus aureus 16 (MRSA16). P5W30 showed a synergistic antibacterial effect with the antibiotic cefoxitin and chloramphenicol against MRSA16. Moreover, the antibiofilm activity of P5W30 was more pronounced when used individually, in comparison with the combination with the antibiotic cefoxitin. Finally, the antiviral activity of P5W30 was tested using the coliphage Qβ, showing a dose-dependent response. The maximum inactivation was observed at 750 μM (6.23 mg·mL−1). In sum, P5W30 shows anti-quorum sensing and antibiofilm activities besides being a potent antibacterial agent against S. aureus and to exhibit antiviral activities against enteric viruses.pt_PT
dc.description.sponsorshipThis research was funded by FCT—Foundation for Science and Technology—through project UIDB/04326/2020 (M.A.) and LA/P/0101/2020; the Austrian Science Fund (FWF): P33927 (N.I.G.); P33089 (A.R.); and the University of Vienna (N.I.G. and A.R.). The authors are grateful to Algarve Biomedical Center (AD-ABC) for Open Access Funding.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiology (Basel). 2022 Jun 30;11(7):994. doi: 10.3390/biology11070994pt_PT
dc.identifier.doi10.3390/biology11070994pt_PT
dc.identifier.issn2079-7737
dc.identifier.urihttp://hdl.handle.net/10400.18/8437
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relation.publisherversionhttps://www.mdpi.com/2079-7737/11/7/994pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntibacterial Drugspt_PT
dc.subjectBacterial Resistancept_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectWells-Dawson Polyoxometalatespt_PT
dc.subjectMultiresistantpt_PT
dc.subjectP5W30pt_PT
dc.subjectP2W18pt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleThe Preyssler-Type Polyoxotungstate Exhibits Anti-Quorum Sensing, Antibiofilm, and Antiviral Activitiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage994pt_PT
oaire.citation.titleBiologypt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication8cf705f5-a3a4-48ab-ba6b-64139b6e0fd6
relation.isProjectOfPublication.latestForDiscovery8cf705f5-a3a4-48ab-ba6b-64139b6e0fd6

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