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Multiomics Assessment of Gene Expression in a Clinical Strain of CTX-M-15-Producing ST131 Escherichia coli

2019-05-03Journal article Open access
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dc.contributor.authorPinto, Luís
dc.contributor.authorTorres, Carmen
dc.contributor.authorGil, Concha
dc.contributor.authorNunes-Miranda, Júlio D.
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorBorges, Vítor
dc.contributor.authorGomes, João P.
dc.contributor.authorSilva, Catarina
dc.contributor.authorVieira, Luís
dc.contributor.authorPereira, José E.
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.date.accessioned2020-04-28T14:43:46Z
dc.date.available2020-04-28T14:43:46Z
dc.date.issued2019-05-03
dc.descriptionFree PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31130921/pt_PT
dc.description.abstractExtended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strain C999 was isolated of a Spanish patient with urinary tract infection. Previous genotyping indicated that this strain presented a multidrug-resistance phenotype and carried beta-lactamase genes encoding CTX-M-15, TEM-1, and OXA-1 enzymes. The whole-cell proteome, and the membrane, cytoplasmic, periplasmic and extracellular sub-proteomes of C999 were obtained in this work by two-dimensional gel electrophoresis (2DE) followed by fingerprint sequencing through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). A total of 602 proteins were identified in the different cell fractions, several of which are related to stress response systems, cellular responses, and antibiotic and drug responses, consistent with the multidrug-resistance phenotype. In parallel, whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) was done to identify and quantify the genes present and expressing. The in silico prediction following WGS confirmed our strain as being serotype O25:H4 and sequence type ST131. The presence of proteins related to antibiotic resistance and virulence in an O25:H4-ST131 E. coli clone are serious indicators of the continued threat of antibiotic resistance spread amongst healthcare institutions. On a positive note, a multiomics approach can facilitate surveillance and more detailed characterization of virulent bacterial clones from hospital environments.pt_PT
dc.description.sponsorshipLP was granted a Ph.D. fellowship by Fundação para a Ciência e a Tecnologia and European Social Fund (SFRH/BD/81307/2011). This work was supported by the Associate Laboratory for Green Chemistry – LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2019). Also, this work is a result of the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Microbiol. 2019 May 3;10:831. doi: 10.3389/fmicb.2019.00831. eCollection 2019.pt_PT
dc.identifier.doi10.3389/fmicb.2019.00831pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.18/6539
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationO PROTEOMA DE STRESS DE ENTEROCOCCUS RESISTENTES À VANCOMICINA: AVALIAÇÃO DAS ALTERAÇÕES NO GENOMA POR IDENTIFICAÇÃO E CARACTERIZAÇÃO DE PROTEÍNAS RELACIONADAS C/ RESISTÊNCIA A ANTIBIÓTICOS E VIRULÊNCIA
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.00831/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBacteriapt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectPublic Healthpt_PT
dc.subjectGenomicspt_PT
dc.subjectTranscriptomicspt_PT
dc.subjectProteomicspt_PT
dc.subjectEscherichia colipt_PT
dc.subjectExtended-spectrum beta-lactamasept_PT
dc.subjectUrinary Tract Infectionpt_PT
dc.subjectWhole Genome Sequencingpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleMultiomics Assessment of Gene Expression in a Clinical Strain of CTX-M-15-Producing ST131 Escherichia colipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleO PROTEOMA DE STRESS DE ENTEROCOCCUS RESISTENTES À VANCOMICINA: AVALIAÇÃO DAS ALTERAÇÕES NO GENOMA POR IDENTIFICAÇÃO E CARACTERIZAÇÃO DE PROTEÍNAS RELACIONADAS C/ RESISTÊNCIA A ANTIBIÓTICOS E VIRULÊNCIA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F81307%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.startPage831pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb7caed21-15d0-4aa3-8385-5457b7bfdf53
relation.isProjectOfPublicationd7883dc7-6335-4f26-8bd0-e2ea4bb07c07
relation.isProjectOfPublication.latestForDiscoveryd7883dc7-6335-4f26-8bd0-e2ea4bb07c07

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