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Genetic diversity and antibiotic resistance of Arcobacter butzleri isolated from poultry and slaughterhouse environment in Portugal

2012-09Conference object Open access
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dc.contributor.authorFerreira, Susana
dc.contributor.authorFraqueza, Maria João
dc.contributor.authorQueiroz, João
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2013-02-14T17:59:51Z
dc.date.available2013-02-14T17:59:51Z
dc.date.issued2012-09
dc.description.abstractArcobacter is considered an emerging enteric pathogen, commonly associated with diarrhea, abdominal pain and in some cases with bacteriemia. This genus is widely distributed, with fteen species identi ed to date, of which the most common is Arcobacter butzleri. Arcobacter spp. has been isolated from environmental, animal, food and human samples, but poultry is considered its main reservoir. The extended use of antibiotics for disease control in modern food animal production, leads to a spread of resistant pathogenic bacteria, and Arcobacter spp. is no exception to this rule. In this study, 43 A. butzleri isolates were obtained from poultry and environment samples at a Portuguese slaughterhouse, also three reference strains were included. All isolates were con rmed at species level by multiplex PCR; genomic DNA ngerprints of all isolates were determined using Pulsed Field Gel Electrophoresis (PFGE) after enzymatic digestion with SmaI. Resistance pro les to nine antibiotics were assessed by broth microdilution method. Fifteen unique and 11 common PFGE ngerprints were identi ed among the 43 Arcobacter isolates studied, generating a total of 26 di erent PFGE ngerprints. This data demonstrates the high genetic diversity observed among Arcobacter isolates. Concerning the antibiotic susceptibility, all isolates tested were susceptible to gentamycin and one strain presented resistance to chloramphenicol. In contrast, 24 of the 43 isolates (55.8%) were resistant to cipro oxacin. All the studied isolates presented resistance to multiple antibiotics simultaneously, especially to ampicillin, vancomycin, trimethoprim, piperacillin, cefoperazone and amoxicillin. The results showed that A. butzleri isolated in Portugal presents a high genetic diversity, but also show high levels of resistance to several antimicrobial agents, this fact could represent a potential health hazard for humans through food chain contamination.por
dc.identifier.citationCESAR 2012 Book of Abstracts. 2010:97 (P 40)por
dc.identifier.urihttp://hdl.handle.net/10400.18/1389
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCroatian Microbiological Societypor
dc.subjectArcobacter Butzleripor
dc.subjectPoultrypor
dc.subjectAntibiotic Resistancepor
dc.subjectInfecções Gastrointestinaispor
dc.titleGenetic diversity and antibiotic resistance of Arcobacter butzleri isolated from poultry and slaughterhouse environment in Portugalpor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePrimosten, Croáciapor
oaire.citation.endPage97por
oaire.citation.startPage97por
oaire.citation.titleCentral European Symposium on Antimicrobials and Antimicrobial Resistance (CESAR 2012), 23-26 September 2012por
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor

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