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New insights on antibacterial mode of action of blue-light photoactivated berberine and curcumin-antibiotic combinations against Staphylococcus aureus

2025-02-06Artigo científico Acesso aberto
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dc.contributor.authorGonçalves, Ariana S.C.
dc.contributor.authorFernandes, José R.
dc.contributor.authorSaavedra, Maria José
dc.contributor.authorGuimarães, Nuno M.
dc.contributor.authorPereira, Cristiana
dc.contributor.authorSimões, Manuel
dc.contributor.authorBorges, Anabela
dc.date.accessioned2026-02-23T11:22:14Z
dc.date.available2026-02-23T11:22:14Z
dc.date.issued2025-02-06
dc.description.abstractAntimicrobial photodynamic inactivation (aPDI), using photosensitisers in combination with antibiotics, is a promising multi-target strategy to address antibiotic resistance, particularly in wound infections. This study aimed to elucidate the antibacterial mode of action of combinations of berberine (Ber) or curcumin (Cur) with selected antibiotics (Ber-Ab or Cur-Ab) under blue light irradiation (420 nm) against Staphylococcus aureus, including methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains. Multiple physiological parameters were assessed using complementary assays (fluorometry, epifluorescence microscopy, flame emission and atomic absorption spectroscopy, zeta potential, flow cytometry, and the plate agar method) to examine the effect on ROS production, membrane integrity, DNA damage, motility and virulence factors of S. aureus. Results indicated that blue light photoactivated Ber-Ab and Cur-Ab combinations led to substantial ROS generation, even at low concentrations, causing oxidative stress that severely impacted bacterial membrane integrity (approximately 90 % in MRSA and 40 % in MSSA). Membrane destabilization was further confirmed by elevated intercellular potassium release (≈ 2.00 and 2.40 µg/mL in MRSA and MSSA, respectively). Furthermore, significant DNA damage was observed in both strains (≈ 50 %). aPDI treatment with blue light also reduced S. aureus pathogenicity by impairing motility and inhibiting key virulence factors such as proteases, lipases, and gelatinases, all of which play key roles in the infectious process. Overall, Ber-Ab combinations demonstrated the highest efficacy across all parameters tested, highlighting for the first time the multi-target therapeutic potential of this phytochemical-based aPDI strategy to combat antibiotic-resistant S. aureus infections and improve wound infection treatment outcomes.eng
dc.description.abstractHighlights: - Photoactivated Ber and Cur-Ab combinations demonstrated multi-target activity. - Photoirradiation significantly enhances the activity of all tested combinations. - Blue light-activated Ber and Cur-Ab combinations generate significant ROS levels. - ROS production boosted the activity of combinations against the bacterial indices. - Ber-Ab combinations consistently performed better on all bacterial indices tested.eng
dc.description.sponsorshipThis work was supported by the Project InnovAntiBiofilm (ref. 101157363) financed by European Commission (Horizon-Widera 2023-Acess-02/Horizon-CSA) and national funds through FCT/MCTES (PIDDAC): LEPABE, UIDB/00511/2020 (DOI: 10.54499/UIDB/00511/2020) and UIDP/00511/2020 (DOI:10.54499/UIDP/00511/2020) and ALiCE, LA/P/0045/2020 (DOI: 10.54499/LA/P/0045/2020). Ariana S.C. Gonçalves acknowledges individual PhD fellowships from FCT (2022.10913.BD).
dc.identifier.citationPhotodiagnosis Photodyn Ther. 2025 Apr:52:104514. doi: 10.1016/j.pdpdt.2025.104514. Epub 2025 Feb 6
dc.identifier.doi10.1016/j.pdpdt.2025.104514
dc.identifier.issn1572-1000
dc.identifier.pmid39920956
dc.identifier.urihttp://hdl.handle.net/10400.18/10983
dc.language.isoen
dc.peerreviewedyes
dc.publisherElsevier
dc.relationBoosting Sustainable Innovation in Developing New Antibiotic Adjuvants to Control Biofilm Resistance
dc.relationUIDB/00511/2020
dc.relationLaboratory for Process Engineering, Environment, Biotechnology and Energy
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1572100025000444?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAntibiotic Resistance
dc.subjectAntimicrobial Photodynamic Inactivation
dc.subjectBerberine/Curcumin-antibiotic Combinations
dc.subjectMulti-target
dc.subjectOxidative Stress
dc.subjectPhotodynamic Therapy
dc.subjectS. aureus Wound Infections
dc.subjectStaphylococcus aureus
dc.subjectResistência aos Antimicrobianos
dc.subjectAgentes Microbianos e Ambiente
dc.titleNew insights on antibacterial mode of action of blue-light photoactivated berberine and curcumin-antibiotic combinations against Staphylococcus aureuseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber101157363
oaire.awardNumberUIDP/00511/2020
oaire.awardTitleBoosting Sustainable Innovation in Developing New Antibiotic Adjuvants to Control Biofilm Resistance
oaire.awardTitleLaboratory for Process Engineering, Environment, Biotechnology and Energy
oaire.awardURIhttp://hdl.handle.net/10400.18/10982
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00511%2F2020/PT
oaire.citation.startPage104514
oaire.citation.titlePhotodiagnosis and Photodynamic Therapy
oaire.citation.volume52
oaire.fundingStreamHORIZON Coordination and Support Actions
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication4141c5f9-b7d6-499f-9cb4-be8cf71cb040
relation.isProjectOfPublication3210f5e0-5b59-47f4-b18d-c3b0fb4e5a93
relation.isProjectOfPublication.latestForDiscovery4141c5f9-b7d6-499f-9cb4-be8cf71cb040

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