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Characterization of AreABC, an RND-Type Efflux System Involved in Antimicrobial Resistance of Aliarcobacter butzleri

2021-08-17Journal article Open access
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dc.contributor.authorFerreira, Susana
dc.contributor.authorSilva, Ana L.
dc.contributor.authorTomás, Joana
dc.contributor.authorMateus, Cristiana
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2022-07-03T15:58:55Z
dc.date.available2022-07-03T15:58:55Z
dc.date.issued2021-08-17
dc.description.abstractAliarcobacter butzleri is an emergent enteropathogen for which resistance to several classes of antimicrobial agents has been described, although the underlying mechanisms have been poorly addressed. We aimed to evaluate the contribution of the resistance-nodulation-division-type (RND) efflux system, AreABC, to drug resistance in A. butzleri. A. butzleri strains were first tested against several antimicrobials with and without an efflux pump inhibitor. Then, erythromycin-resistant strains were screened for the presence of a premature stop codon in a putative transcriptional regulator of the AreABC system, areR. Lastly, antimicrobial susceptibility and ethidium bromide (EtBr) accumulation were evaluated using an areB knockout strain and a strain overexpressing the AreABC system through areR truncation. The presence of the efflux pump inhibitor resulted in increased susceptibility to most of the antimicrobials tested. A correlation between erythromycin resistance and the presence of premature stop codons in areR was observed. The truncation of areR resulted in increased expression of the AreABC system and decreased susceptibility to various antimicrobials. In contrast, areB inactivation resulted in increased susceptibility and a higher intracellular accumulation of EtBr. In conclusion, the AreABC efflux pump plays a role in the resistance of A. butzleri to multiple drugs and is regulated by a putative transcriptional repressor, areR. Our results support the importance of efflux pumps in this bacterium's resistance to major classes of antibiotics and other antimicrobials.pt_PT
dc.description.sponsorshipSusana Ferreira acknowledges the Universidade da Beira Interior (UBI) and the Foundation for Science and Technology (FCT), both involved in the scientific employment contract according to DL57/2016. Cristiana Mateus acknowledges the fellowship reference BID/ICI-FCS/Santander Universidades-UBI/2019 within the scope of the protocol signed between the UBI and Santander Universidades. Sanger sequencing was performed at the Unidade de Tecnologia e Inovação (Departamento de Genética Humana, Instituto Nacional de Saúde Ricardo Jorge, Lisbon, Portugal). This work was supported by the Foundation for Science and Technology (FCT) through funds from the state budget and by the European Regional Development Fund (ERDF) under the Portugal 2020 Program through the Regional Operational Program of the Center (Centro2020) and through the project with the reference UIDB/00709/2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntimicrob Agents Chemother. 2021 Aug 17;65(9):e0072921. doi: 10.1128/AAC.00729-21. Epub 2021 Aug 17pt_PT
dc.identifier.doi10.1128/AAC.00729-21pt_PT
dc.identifier.issn0066-4804
dc.identifier.urihttp://hdl.handle.net/10400.18/8050
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationNot Available
dc.relationHealth Sciences Research Centre
dc.relation.publisherversionhttps://journals.asm.org/doi/10.1128/AAC.00729-21pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAliarcobacter butzleript_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectEfflux Pumppt_PT
dc.subjectResistance Nodulation Cell Divisionpt_PT
dc.subjectTranscriptional Repressorpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleCharacterization of AreABC, an RND-Type Efflux System Involved in Antimicrobial Resistance of Aliarcobacter butzleript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL57%2F2016%2FCP1334%2FCP1644%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.citation.issue9pt_PT
oaire.citation.startPagee0072921pt_PT
oaire.citation.titleAntimicrobial Agents and Chemotherapypt_PT
oaire.citation.volume65pt_PT
oaire.fundingStreamDL 57/2016
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationcd8d582a-84fb-439a-a2c7-aa4ec69e78b4
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublication.latestForDiscoveryced21566-cfde-4640-afff-0536a07c1f8f

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