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Global DNA methylation and Reactive Oxygen Species assessment after in vitro exposure to novel cellulose nanomaterials on intestinal cells

2023-12-01Conference object Restricted access
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dc.contributor.authorVital, Nádia
dc.contributor.authorVentura, Célia
dc.contributor.authorKranendonk, M.
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2024-01-29T16:26:46Z
dc.date.available2024-01-29T16:26:46Z
dc.date.issued2023-12-01
dc.descriptionThanks to P.Alvito, R.Assunção and M.Silva(Food Safety Department, INSA) for support with the in vitro digestion.pt_PT
dc.descriptionAbstract disponível em: https://omp.uv.es/index.php/PUV/catalog/book/537pt_PT
dc.description.abstractInnovative cellulose nanomaterials (CNMs) find applications in multiple food-related products. However, it is recognized that nanomaterials (NMs) may cause adverse effects frequently by biological mechanisms that include production of intrinsic reactive oxygen species (ROS), or epigenetic alterations, by changes in gene expression through alterations in DNA methylation. To contribute to the safety assessment of two types of micro/nanofibrillated celluloses (CMF/CNFs) derived from industrial Eucalyptus globulus kraft, intracellular ROS levels and global DNA methylation patterns were evaluated using human intestinal cell models exposed to these CNMs. A harmonized protocol for in vitro simulation of human digestion was applied to incorporate the effect of potential changes in the physicochemical properties of CNMs due to the digestion process. After exposure of intestinal cells to digested and undigested CNMs samples (concentrations from 3.1 to 200 μg/mL, 3h and 24h), ROS levels were determined using the 2,7-dichlorofluorescein diacetate probe and fluorescence detection. The global DNA methylation was assessed after 24h exposure, using the methylated DNA Quantification Kit(colorimetric, Abcam) to quantify the levels of methylated cytosines (5-methylcytosine) in CpG dinucleotides. No increased ROS were observed after exposure to CNMs with and without digestion. Conversely, the preliminary results showed significant increased methylation of DNA after exposure to the CMF, without digestion, as compared to the negative control. No effect could be observed for digested sample, possibly due to the high background of the digestion product per se. Our preliminary findings seem to suggest the occurrence of biological effects of CNMs in intestinal cells that require further investigation, as may lead to a toxic impact on gastrointestinal cells, of concern for human health.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through national funds (PTDC/SAU-PUB/29481/2017; PTDC/SAUPUB/ 32587/2017 and UIDB/00009/2020; UIDP/00009/2020); NV holds a FCT/MCTES PhD grant 2020.07168.BD.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8990
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectReactive Oxygens Speciespt_PT
dc.subjectGastrointestinal Effectspt_PT
dc.subjectGlobal DNA Methylationpt_PT
dc.subjectCellulose Nanofibrespt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleGlobal DNA methylation and Reactive Oxygen Species assessment after in vitro exposure to novel cellulose nanomaterials on intestinal cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.citation.conferencePlaceValencia, Espanhapt_PT
oaire.citation.titleRiskTox - Networking on food safety and innovative techniques for evaluating human health risks: II International Workshop, 1 December 2023pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVital
person.familyNameVentura
person.familyNameKranendonk
person.familyNameSilva
person.familyNameLouro
person.givenNameNádia
person.givenNameCélia
person.givenNameMichel
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier2056460
person.identifier117538
person.identifier157627
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-idFC1B-515E-3C03
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-3391-0268
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0003-1362-0076
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.ridH-8176-2013
person.identifier.scopus-author-id6603648823
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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