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Pathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterization

2022-10-17Journal article Open access
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dc.contributor.authorPista, Angela
dc.contributor.authorSilveira, Leonor
dc.contributor.authorRibeiro, Sofia
dc.contributor.authorFontes, Mariana
dc.contributor.authorCastro, Rita
dc.contributor.authorCoelho, Anabela
dc.contributor.authorFurtado, Rosália
dc.contributor.authorLopes, Teresa
dc.contributor.authorMaia, Carla
dc.contributor.authorMixão, Verónica
dc.contributor.authorBorges, Vítor
dc.contributor.authorSá, Ana
dc.contributor.authorSoeiro, Vanessa
dc.contributor.authorCorreia, Cristina Belo
dc.contributor.authorGomes, João Paulo
dc.contributor.authorSaraiva, Margarida
dc.contributor.authorOleastro, Mónica
dc.contributor.authorBatista, Rita
dc.date.accessioned2023-01-12T10:34:55Z
dc.date.available2023-01-12T10:34:55Z
dc.date.issued2022-10-17
dc.descriptionThis article belongs to the Section Food Microbiology.pt_PT
dc.description.abstractHuman–wildlife coexistence may increase the potential risk of direct transmission of emergent or re-emergent zoonotic pathogens to humans. Intending to assess the occurrence of three important foodborne pathogens in wild animals of two wildlife conservation centers in Portugal, we investigated 132 fecal samples for the presence of Escherichia coli (Shiga toxin-producing E. coli (STEC) and non-STEC), Salmonella spp. and Campylobacter spp. A genotypic search for genes having virulence and antimicrobial resistance (AMR) was performed by means of PCR and Whole-Genome Sequencing (WGS) and phenotypic (serotyping and AMR profiles) characterization. Overall, 62 samples tested positive for at least one of these species: 27.3% for STEC, 11.4% for non-STEC, 3.0% for Salmonella spp. and 6.8% for Campylobacter spp. AMR was detected in four E. coli isolates and the only Campylobacter coli isolated in this study. WGS analysis revealed that 57.7% (30/52) of pathogenic E. coli integrated genetic clusters of highly closely related isolates (often involving different animal species), supporting the circulation and transmission of different pathogenic E. coli strains in the studied areas. These results support the idea that the health of humans, animals and ecosystems are interconnected, reinforcing the importance of a One Health approach to better monitor and control public health threats.pt_PT
dc.description.sponsorshipThis work was supported by funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 773830: One Health European Joint Pro- gramme, as part of the DiSCoVeR project (Discovering the sources of Salmonella, Campylobacter, VTEC and Antimicrobial Resistance). S.R., R.C. and V.M. were beneficiaries of fellowships from the same Programme on behalf of ADONIS (S.R.), FedAMR (R.C.) and BeOne (V.M) projects.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms . 2022 Oct 27;10(11):2132. doi: 10.3390/microorganisms10112132pt_PT
dc.identifier.doi10.3390/microorganisms10112132pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/8430
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/10/11/2132pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPathogenic Escherichia colipt_PT
dc.subjectSalmonella spp.pt_PT
dc.subjectCampylobacter spp.pt_PT
dc.subjectWhole Genome Sequencingpt_PT
dc.subjectWild Animalspt_PT
dc.subjectPortugalpt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titlePathogenic Escherichia coli, Salmonella spp. and Campylobacter spp. in Two Natural Conservation Centers of Wildlife in Portugal: Genotypic and Phenotypic Characterizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/773830/EU
oaire.citation.issue11pt_PT
oaire.citation.startPage2132pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication94d118fb-33ce-49fa-b1ed-d5bddf63581d
relation.isProjectOfPublication.latestForDiscovery94d118fb-33ce-49fa-b1ed-d5bddf63581d

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