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Nontuberculous Mycobacteria Persistence in a Cell Model Mimicking Alveolar Macrophages

2019-04-26Journal article Open access
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dc.contributor.authorSousa, Sara
dc.contributor.authorBorges, Vítor
dc.contributor.authorJoão, Inês
dc.contributor.authorGomes, João Paulo
dc.contributor.authorJordão, Luisa
dc.date.accessioned2019-05-30T09:43:12Z
dc.date.available2019-05-30T09:43:12Z
dc.date.issued2019-04-26
dc.description.abstractNontuberculous Mycobacteria (NTM) respiratory infections have been gradually increasing. Here, THP-1 cells were used as a model to evaluate intracellular persistence of three NTM species (reference and clinical strains) in human alveolar macrophages. The contribution of phagosome acidification, nitric oxide (NO) production and cell dead on NTM intracellular fate was assessed. In addition, strains were characterized regarding their repertoire of virulence factors by whole-genome sequencing. NTM experienced different intracellular fates: M. smegmatis and M. fortuitum ATCC 6841 were cleared within 24h. In contrast, M. avium strains (reference/clinical) and M. fortuitum clinical strain were able to replicate. Despite this fact, unexpectedly high percentages of acidified phagosomes were found harbouring rab7, but not CD63. All NTM were able to survive in vitro at acidic pHs, with the exception of M. smegmatis. Our data further suggested a minor role for NO in intracellular persistence and that apoptosis mediated by caspase 8 and 3/7, but not necrosis, is triggered during NTM infection. Insights regarding the bacteria genomic backbone corroborated the virulence potential of M. avium and M. fortuitum. In conclusion, the phenotypic traits detected contrast with those described for M. tuberculosis, pointing out that NTM adopt distinct strategies to manipulate the host immune defense and persist intracellularly.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2019 Apr 26;7(5). pii: E113. doi: 10.3390/microorganisms7050113pt_PT
dc.identifier.doi10.3390/microorganisms7050113pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/6411
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/7/5/113pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNontuberculous Mycobacteriapt_PT
dc.subjectHost-pathogen Interactionpt_PT
dc.subjectMacrophagespt_PT
dc.subjectpHpt_PT
dc.subjectVirulencept_PT
dc.subjectNTM Infectionpt_PT
dc.subjectRespiratory Infectionspt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.subjectAgentes Microbianos e Ambientept_PT
dc.titleNontuberculous Mycobacteria Persistence in a Cell Model Mimicking Alveolar Macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceBaselpt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPagepii: E113pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume7pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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