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Quantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursa

2020-02-19Journal article Open access
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dc.contributor.authorCouto, Joana
dc.contributor.authorVillar, Margarita
dc.contributor.authorMateos-Hernández, Lourdes
dc.contributor.authorFerrolho, Joana
dc.contributor.authorSanches, Gustavo
dc.contributor.authorSofia Santos, Ana
dc.contributor.authorSantos-Silva, Maria
dc.contributor.authorNobre, João
dc.contributor.authorMoreira, Olga
dc.contributor.authorAntunes, Sandra
dc.contributor.authorde la Fuente, José
dc.contributor.authorDomingos, Ana
dc.date.accessioned2021-04-05T17:08:49Z
dc.date.available2021-04-05T17:08:49Z
dc.date.issued2020-02-19
dc.description.abstractThe negative impact of ticks and tick-borne diseases on animals and human health is driving research to discover novel targets affecting both vectors and pathogens. The salivary glands are involved in feeding and pathogen transmission, thus are considered as a compelling target to focus research. In this study, proteomics approach was used to characterize Rhipicephalusbursa sialoproteome in response to Babesiaovis infection and blood feeding. Two potential tick protective antigens were identified and its influence in tick biological parameters and pathogen infection was evaluated. Results demonstrate that the R. bursa sialoproteome is highly affected by feeding but infection is well tolerated by tick cells. The combination of both stimuli shifts the previous scenario and a more evident pathogen manipulation can be suggested. Knockdown of ub2n led to a significative increase of infection in tick salivary glands but a brusque decrease in the progeny, revealing its importance in the cellular response to pathogen infection, which is worth pursuing in future studies. Additionally, an impact in the recovery rate of adults (62%), the egg production efficiency (45.75%), and the hatching rate (88.57 %) was detected. Building knowledge on vector and/or pathogen interplay bridges the identification of protective antigens and the development of novel control strategies.pt_PT
dc.description.sponsorshipThis research was funded by Fundação para a Ciência e Tecnologia (FCT) under the project TickOmic (PTDC/CVT-CVT/29073/2017). JC and JF are the recipients of Ph.D. grants supported by the FCT (SFRH/BD/121946/2016, SFRH/BD/122894/2016, respectively).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVaccines (Basel). 2020 Feb 19;8(1):91. doi: 10.3390/vaccines8010091.pt_PT
dc.identifier.doi10.3390/vaccines8010091pt_PT
dc.identifier.issn2076-393X
dc.identifier.urihttp://hdl.handle.net/10400.18/7640
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationVector-pathogen interactomics: connecting the dots using tick cell lines
dc.relationA proteomic and functional genomic insight into the Rhipicephalus sanguineus-Ehrlichia canis interactome towards vaccine development
dc.relation.publisherversionhttps://www.mdpi.com/2076-393X/8/1/91pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBabesiapt_PT
dc.subjectPCCApt_PT
dc.subjectRNAipt_PT
dc.subjectUB2Npt_PT
dc.subjectProteomicpt_PT
dc.subjectTickspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleQuantitative Proteomics Identifies Metabolic Pathways Affected by Babesia Infection and Blood Feeding in the Sialoproteome of the Vector Rhipicephalus bursapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVector-pathogen interactomics: connecting the dots using tick cell lines
oaire.awardTitleA proteomic and functional genomic insight into the Rhipicephalus sanguineus-Ehrlichia canis interactome towards vaccine development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-CVT%2F29073%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F121946%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F122894%2F2016/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage91pt_PT
oaire.citation.titleVaccinespt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication2e338f32-d435-4c36-b668-dc90c37b88a7
relation.isProjectOfPublicatione6ecf2d6-329a-42e3-8ab5-8eb0d7ff48d4
relation.isProjectOfPublicationddedb8ee-edca-4feb-b67f-3487446f8d4a
relation.isProjectOfPublication.latestForDiscovery2e338f32-d435-4c36-b668-dc90c37b88a7

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