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Proteomic and Real-Time PCR analyses of Saccharomyces cerevisiae VL3 exposed to microcystin-LR reveals a set of protein alterations transversal to several eukaryotic models

2016-03-15Journal article Restricted access
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dc.contributor.authorValério, Elisabete
dc.contributor.authorCampos, Alexandre
dc.contributor.authorOsório, Hugo
dc.contributor.authorVasconcelos, Vitor
dc.date.accessioned2017-03-07T16:42:55Z
dc.date.available2021-01-22T01:30:11Z
dc.date.issued2016-03-15
dc.description.abstractSome of the most common toxins present in freshwater, in particular microcystins (MCs), are produced by cyanobacteria. These toxins have a negative impact on human health, being associated with episodes of acute hepatotoxicity and being considered potentially carcinogenic to humans. To date the exact mechanisms of MC-induced toxicity and tumor promotion were not completely elucidated. To get new insights underlying microcystin-LR (MCLR) molecular mechanisms of toxicity we have performed the proteomic profiling using two-dimensional electrophoresis and MALDI-TOF/TOF of Saccharomyces cerevisiae cells exposed for 4 h-1 nM and 1 μM of MCLR, and compared them to the control (cells not exposed to MCLR). We identified 14 differentially expressed proteins. The identified proteins are involved in metabolism, genotoxicity, cytotoxicity and stress response. Furthermore, we evaluated the relative expression of yeast's PP1 and PP2A genes and also of genes from the Base Excision Repair (BER) DNA-repair system, and observed that three out of the five genes analyzed displayed dose-dependent responses. Overall, the different proteins and genes affected are related to oxidative stress and apoptosis, thus reinforcing that it is probably the main mechanism of MCLR toxicity transversal to several organisms, especially at lower doses. Notwithstanding these MCLR responsive proteins could be object of further studies to evaluate their suitability as biomarkers of exposure to the toxin.pt_PT
dc.description.sponsorshipThis research was partially supported by the European Regional Development Fund (ERDF) through the COMPETE e Operational Competitiveness Program and national funds through FCT (Foundation for Science and Technology) grants to E. Val erio (SFRH/BPD/ 75922/2011) and A. Campos (SFRH/BPD/103683/2014) and through the project PEst-C/MAR/LA0015/2013.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationToxicon. 2016 Mar 15;112:22-8. doi: 10.1016/j.toxicon.2016.01.059. Epub 2016 Jan 21pt_PT
dc.identifier.doi10.1016/j.toxicon.2016.01.059pt_PT
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10400.18/4553
dc.language.isoporpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ International Society on Toxinologypt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0041010116300101pt_PT
dc.subjectApoptosispt_PT
dc.subjectBacterial Toxinspt_PT
dc.subjectDNA Repairpt_PT
dc.subjectGene Expression Profilingpt_PT
dc.subjectGene Expression Regulation, Bacterialpt_PT
dc.subjectMarine Toxinspt_PT
dc.subjectMicrocystinspt_PT
dc.subjectOsmolar Concentrationpt_PT
dc.subjectOxidative Stresspt_PT
dc.subjectProteomicspt_PT
dc.subjectReal-Time Polymerase Chain Reactionpt_PT
dc.subjectReverse Transcriptase Polymerase Chain Reactionpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectSaccharomyces cerevisiae Proteinspt_PT
dc.subjectSpecies Specificitypt_PT
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationpt_PT
dc.subjectTwo-Dimensional Difference Gel Electrophoresispt_PT
dc.subjectvpt_PT
dc.titleProteomic and Real-Time PCR analyses of Saccharomyces cerevisiae VL3 exposed to microcystin-LR reveals a set of protein alterations transversal to several eukaryotic modelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FMAR%2FLA0015%2F2013/PT
oaire.citation.endPage28pt_PT
oaire.citation.startPage22pt_PT
oaire.citation.titleToxiconpt_PT
oaire.citation.volume112pt_PT
oaire.fundingStreamCOMPETE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication93b38b87-6e5d-4154-b69f-355e4ee7efaf
relation.isProjectOfPublication.latestForDiscovery93b38b87-6e5d-4154-b69f-355e4ee7efaf

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