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In vitro genotoxicity assessment of an oxidized dextrin-based hydrogel for biomedical applications

2018-11-28Journal article Restricted access
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dc.contributor.authorPereira, Isabel
dc.contributor.authorFraga, Sónia
dc.contributor.authorSilva, Susana
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorGama, Miguel
dc.date.accessioned2019-03-06T12:09:51Z
dc.date.available2019-03-06T12:09:51Z
dc.date.issued2018-11-28
dc.description.abstractHydrogels are three‐dimensional, crosslinked networks of hydrophilic polymers swollen with a large amount of water or biological fluids, without dissolving. Dextrin, a low‐molecular‐weight carbohydrate composed by glucose residues, has been used to develop an injectable hydrogel for biomedical applications. Dextrin was first oxidized to introduce aldehyde groups, which then reticulate with adipic acid dihydrazide, forming the dextrin‐based hydrogel (HG). The HG and its components were tested for cyto‐ and genotoxicity according to the International Standard ISO 10993‐3 on the biological evaluation of medical devices. To assess genotoxicity, a battery of in vitro genotoxicity tests employing both eukaryotic and prokaryotic models was performed: comet assay, cytokinesis‐block micronucleus assay and Ames test. Our data revealed that the HG (IC50 = 2.8 mg/mL) and oxidized dextrin by itself (IC50 = 1.2 mg/mL) caused a concentration‐dependent decrease in cellular viability of human lymphoblastoid TK6 cells after 24 hours of exposure to the test agents. However, these concentrations are unlikely to be reached in vivo. In addition, no significant increase in the DNA and chromosomal damage of TK6 cells exposed to non‐cytotoxic concentrations of the HG and its isolated components was detected. Furthermore, neither the HG nor its metabolites exerted a mutagenic effect in different of Salmonella typhimurium strains and in an Escherichia coli mix. Our data demonstrated the genocompatibility of the HG (up to 3.5 mg/mL) for biomedical applications. To our best acknowledge, this is the first report with a detailed genotoxicity assessment of an aldehyde‐modified polysaccharide/adipic acid dihydrazide hydrogel.pt_PT
dc.description.sponsorshipFEDER ‐ Fundo Europeu de Desenvolvimento Regional, Grant/Award Numbers: NORTE‐01‐ 0145‐FEDER‐000004 and NORTE‐01‐0247‐FEDER‐003262; Fundação para a Ciência e a Tecnologia, Grant/Award Numbers: PTDC/CTM‐BIO/2170/2014, SFRH/BD/90066/2012pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Appl Toxicol. 2018 Nov 28. doi: 10.1002/jat.3754. [Epub ahead of print]pt_PT
dc.identifier.doi10.1002/jat.3754pt_PT
dc.identifier.issn0260-437X
dc.identifier.urihttp://hdl.handle.net/10400.18/6069
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley and Sonspt_PT
dc.relationDEXTRIN INJECTABLE HYDROGEL FOR TISSUE HEALING AND REGENERATION
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/jat.3754pt_PT
dc.subjectAmes Testpt_PT
dc.subjectTK6 Cellspt_PT
dc.subjectAdipic Acid Dihydrazide (ADH)pt_PT
dc.subjectAldehyde-modified Polysaccharidept_PT
dc.subjectComet Assaypt_PT
dc.subjectCytokinesis-block Micronucleus (CBMN) Assaypt_PT
dc.subjectDextrinpt_PT
dc.subjectHydrogelpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleIn vitro genotoxicity assessment of an oxidized dextrin-based hydrogel for biomedical applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEXTRIN INJECTABLE HYDROGEL FOR TISSUE HEALING AND REGENERATION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F90066%2F2012/PT
oaire.citation.titleJournal of Applied Toxicologypt_PT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationdabf6315-4fcd-4c1a-9766-751328fca4c3
relation.isProjectOfPublication.latestForDiscoverydabf6315-4fcd-4c1a-9766-751328fca4c3

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