Publicação
Genetic variants in red blood cell adhesion-related genes influence the severity of sickle cell anemia in a malaria-endemic region
| datacite.subject.fos | Ciências Médicas | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Matos, Irina | |
| dc.contributor.author | Santos, Brígida | |
| dc.contributor.author | Gonçalves, Elisângela | |
| dc.contributor.author | Lopes, Pedro | |
| dc.contributor.author | Brito, Miguel | |
| dc.contributor.author | Arez, Ana Paula | |
| dc.contributor.author | Faustino, Paula | |
| dc.date.accessioned | 2026-10-01T10:38:20Z | |
| dc.date.available | 2026-10-01T10:38:20Z | |
| dc.date.issued | 2026-05-28 | |
| dc.description.abstract | Background: Sickle cell anemia (SCA) is a genetic disease marked by abnormal hemoglobin S and sickle-shaped red blood cells. It is highly prevalent in sub-Saharan Africa, especially in Angola, where SCA and malaria are major causes of childhood mortality. This study aimed to explore whether genetic variants in genes associated with red blood cell adhesion to the vascular endothelium influence the manifestations of SCA in Angolan pediatric patients in the context of malaria. Methods and results: The study enrolled 65 pediatric SCA patients living in Luanda or Caxito. Their clinical, hematological, and biochemical profiles were monitored through longitudinal pediatric follow-up appointments. Fifteen polymorphic sites were genotyped in CD36 and ICAM-1 genes using PCR, Sanger sequencing, and fragment analysis by capillary electrophoresis. Malaria infection was evaluated by detecting Plasmodium species DNA through PCR analysis of blood spot samples. The CD36 variant rs3211891_C is revealed for the first time as a potential modulator of anemia severity in SCA. Additionally, the CD36 variant rs3211938_G, along with the ICAM-1 variants rs5491_T and rs5496_A, significantly impacted the severity of the hematological phenotype in SCA. Furthermore, SCA patients carrying the ICAM-1 rs5494_T variant showed a 5.63-fold increased risk of having malaria infection compared to those with the wild-type genotype. Conclusions: This study enhances our understanding of genetic modifiers of red blood cell adhesion to the vascular endothelium and their influence on the severity of pediatric SCA in the context of frequent concomitant malaria infection in Angola. | eng |
| dc.description.sponsorship | Open access funding provided by FCT|FCCN (b-on). This work was partially supported by the Fundação Para a Ciência e a Tecnologia (FCT, Portugal)/Aga Khan Development Network, Grant number 330842553, GHTM-UID/04413/2025 and LA-REAL—LA/P/0117/2020. | |
| dc.identifier.citation | Mol Biol Rep. 2026 May 28;53(1):855. doi: 10.1007/s11033-026-12029-w | |
| dc.identifier.doi | 10.1007/s11033-026-12029-w | |
| dc.identifier.eissn | 1573-4978 | |
| dc.identifier.issn | 0301-4851 | |
| dc.identifier.pmid | 42207357 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/11361 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Springer | |
| dc.relation | Translation and Innovation towards Global Health | |
| dc.relation | GHTM-UID/04413/2025 | |
| dc.relation | LA/P/0117/2020 | |
| dc.relation.hasversion | https://link.springer.com/article/10.1007/s11033-026-12029-w | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | CD36 | |
| dc.subject | ICAM-1 | |
| dc.subject | Angola | |
| dc.subject | Genetic Modifiers | |
| dc.subject | Malaria | |
| dc.subject | Sickle Cell Anemia | |
| dc.subject | Doenças Genéticas | |
| dc.subject | Drepanocitose | |
| dc.subject | Anemia Falciforme | |
| dc.subject | Factores Genéticos Modificadores | |
| dc.title | Genetic variants in red blood cell adhesion-related genes influence the severity of sickle cell anemia in a malaria-endemic region | eng |
| dc.title.alternative | Genetic variants in red blood cell adhesion-related genes in sickle cell anemia | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | LA/P/0117/2020 | |
| oaire.awardTitle | Translation and Innovation towards Global Health | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0117%2F2020/PT | |
| oaire.citation.issue | 1 | |
| oaire.citation.startPage | 855 | |
| oaire.citation.title | Molecular Biology Reports | |
| oaire.citation.volume | 53 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| relation.isProjectOfPublication | 8fda9aa0-466f-48d1-8017-ebb8180fc3ae | |
| relation.isProjectOfPublication.latestForDiscovery | 8fda9aa0-466f-48d1-8017-ebb8180fc3ae |