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Evaluation of the cytotoxic and genotoxic effects of benchmark multi-walled carbon nanotubes in relation to their physicochemical properties

2016-10-01Journal article Restricted access
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dc.contributor.authorLouro, Henriqueta
dc.contributor.authorPinhão, Mariana
dc.contributor.authorSantos, Joana
dc.contributor.authorTavares, Ana
dc.contributor.authorVital, Nádia
dc.contributor.authorSilva, Maria João
dc.date.accessioned2016-10-13T13:45:03Z
dc.date.available2020-10-01T00:30:11Z
dc.date.issued2016-10-01
dc.description.abstractTo contribute with scientific evidence to the grouping strategy for the safety assessment of multi-walled carbon nanotubes (MWCNTs), this work describes the investigation of the cytotoxic and genotoxic effects of four benchmark MWCNTs in relation to their physicochemical characteristics, using two types of human respiratory cells. The cytotoxic effects were analysed using the clonogenic assay and replication index determination. A 48h-exposure of cells revealed that NM-401 was the only cytotoxic MWCNT in both cell lines, but after 8-days exposure, the clonogenic assay in A549 cells showed cytotoxic effects for all the tested MWCNTs. Correlation analysis suggested an association between the MWCNTs size in cell culture medium and cytotoxicity. No induction of DNA damage was observed after any MWCNTs in any cell line by the comet assay, while the micronucleus assay revealed that both NM-401 and NM-402 were genotoxic in A549 cells. NM-401 and NM-402 are the two longest MWCNTs analyzed in this work, suggesting that length may be determinant for genotoxicity. No induction of micronuclei was observed in Beas-2B cell line and the different effect in both cell lines is explained in view of the size-distribution of MWCNTs in the cell culture medium, rather than cell's specificities.pt_PT
dc.description.sponsorshipThe research leading to these results was co-funded by European Union Seventh Framework Programme (FP7/2007e2013) under the project NANoREG (A common European approach to the regulatory testing of nanomaterials), grant agreement 310584, by NANOGENOTOX Joint Action, in the framework of the Health Programme under grant agreement 2009 21 and by UID/BIM/00009/2013 (Centre for Toxicogenomics and Human Health – ToxOmics, Foundation for Science and Technology). The authors also acknowledge Dr. José Catita (Paralab, Portugal) for the support with the DLS analysis.pt_PT
dc.identifier.citationToxicol Lett. 2016 Oct 1;262:123-134. doi: 10.1016/j.toxlet.2016.09.016pt_PT
dc.identifier.doi10.1016/j.toxlet.2016.09.016pt_PT
dc.identifier.issn0378-4274
dc.identifier.urihttp://hdl.handle.net/10400.18/4046
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/EUROTOXpt_PT
dc.relationNANOGENOTOX Joint Action, in the framework of the Health Programme under grant agreement 2009 21pt_PT
dc.relationA common European approach to the regulatory testing of nanomaterials
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378427416332507pt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectNanomaterialspt_PT
dc.subjectMulti-walled Carbon Nanotubespt_PT
dc.subjectHuman Respiratory Cellspt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectCarbon Nanotubespt_PT
dc.subjectPhysicochemical Characteristicspt_PT
dc.titleEvaluation of the cytotoxic and genotoxic effects of benchmark multi-walled carbon nanotubes in relation to their physicochemical propertiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleA common European approach to the regulatory testing of nanomaterials
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/310584/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.citation.endPage134pt_PT
oaire.citation.startPage123-134pt_PT
oaire.citation.titleToxicology Letterspt_PT
oaire.citation.volume262pt_PT
oaire.fundingStreamFP7
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf9fe5c15-606f-4958-ac4b-8109981d34c7
relation.isProjectOfPublicatione9cc9728-4f09-4e3a-b30d-53d4429986fb
relation.isProjectOfPublication.latestForDiscoveryf9fe5c15-606f-4958-ac4b-8109981d34c7

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