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Serological and molecular evidence of Bartonella henselae in cats from Luanda city, Angola

2019-04-14Journal article Restricted access
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dc.contributor.authorBarradas, P.F.
dc.contributor.authorDe Sousa, R.
dc.contributor.authorVilhena, H.
dc.contributor.authorOliveira, A.C.
dc.contributor.authorLuz, M.F.
dc.contributor.authorGranada, S.
dc.contributor.authorCardoso, L.
dc.contributor.authorLopes, A.P.
dc.contributor.authorGonçalves, H.
dc.contributor.authorMesquita, J.R.
dc.contributor.authorFerreira, P.
dc.contributor.authorAmorim, I.
dc.contributor.authorGärtner, F.
dc.date.accessioned2020-04-29T06:04:18Z
dc.date.available2020-04-29T06:04:18Z
dc.date.issued2019-04-14
dc.description.abstractA total of 100 domestic cats from Luanda (Angola) were tested for the presence of antibodies against Bartonella henselae and spotted fever group of Rickettsia (SFGR) using indirect immunofluorescence assay (IFA). Molecular screening targeting the riboflavin synthase (ribC) gene for Bartonella and outer membrane protein B (ompB) gene for Rickettsia, using conventional PCR and sequencing was also performed in cat´s blood samples. Sixty-six percent of the cats from Luanda had IgG antibodies against Bartonella species but none of them had antibodies against SFGR. Of the total seroreactive cats for Bartonella henselae, 4.5% had an IgG titre of 64 (cut-off), 60.6% a titre of 128, 28.8% a titre of 256 and 6.1% a titre of 512. A statistically significant association was observed between seropositivity for Bartonella henselae and the lack of access to prophylaxis against ectoparasites (p = 0.018). Molecular detection and further sequence analysis of the positive amplicons allowed identification of Bartonella henselae in a 2-year old male cat. To the best of our knowledge this study confirms for the first time, the presence of Bartonela henselae circulating in domestic cats from Luanda. This fact call the attention for the possible cases of cat-scratch disease in humans.pt_PT
dc.description.sponsorshipP. Barradas (SFRH/BD/116449/2016) acknowledges FCT, the Portuguese Foundation for Science and Technology for financial support. IPATIMUP integrates the i3S Research Unit, which is partially supported by FEDERthrough the Operational Programme for Competitiveness Factors-COMPETE and National Funds through FCT, under the project number PEst-C/SAU/LA0003/2013. This paper was published under the framework of the European Social Fund, Human Resources Development Operational Programme 2007–2013 (POSDRU/159/1.5/S/136893).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationActa Trop. 2019 Jul;195:142-144. doi: 10.1016/j.actatropica.2019.04.012. Epub 2019 Apr 14pt_PT
dc.identifier.doi10.1016/j.actatropica.2019.04.012pt_PT
dc.identifier.issn0001-706X
dc.identifier.urihttp://hdl.handle.net/10400.18/6555
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S0001706X18313433?via%3Dihubpt_PT
dc.subjectCatpt_PT
dc.subjectBartonella henselaept_PT
dc.subjectSFGRpt_PT
dc.subjectFlea-borne Diseasespt_PT
dc.subjectIFApt_PT
dc.subjectPCRpt_PT
dc.subjectAngolapt_PT
dc.subjectInfecções Sistémicas e Zoonoses
dc.titleSerological and molecular evidence of Bartonella henselae in cats from Luanda city, Angolapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FSAU%2FLA0003%2F2013/PT
oaire.citation.endPage144pt_PT
oaire.citation.startPage142pt_PT
oaire.citation.titleActa Tropicapt_PT
oaire.citation.volume195pt_PT
oaire.fundingStreamCOMPETE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf37b50c3-33c1-479a-a758-f45aa5b3d99f
relation.isProjectOfPublication.latestForDiscoveryf37b50c3-33c1-479a-a758-f45aa5b3d99f

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