Publication
Detection of Dientamoeba fragilis in Portuguese children with acute gastroenteritis between 2011 and 2013
| dc.contributor.author | Júlio, C. | |
| dc.contributor.author | Furtado, Cristina | |
| dc.contributor.author | Rocha, R. | |
| dc.contributor.author | Escobar, C. | |
| dc.contributor.author | Brito, M.J. | |
| dc.contributor.author | Oleastro, Mónica | |
| dc.date.accessioned | 2016-02-26T17:54:09Z | |
| dc.date.available | 2016-04-01T00:30:10Z | |
| dc.date.issued | 2015-09 | |
| dc.description.abstract | Dientamoeba fragilis is an inhabitant of human gastrointestinal tract with a worldwide distribution. The first description considered this protozoan a rare and harmless commensal, since then it has struggled to gain recognition as a pathogen. Commercial multiplex real-time PCR was used to detect D. fragilis in fecal samples from hospitalized children (⩽18 years) with acute gastrointestinal disease, admitted to two hospitals of Lisbon area, with different demographic characteristics. A total of 176 children were studied, 103 (58·5%) male, 144 (81·8%) children between 0 and 5 years and 32 (18·2%) above 6 years old. The overall protozoa frequency considering the four tested microorganisms were 8·5% (15/176), and the most frequently found protozoan was D. fragilis, 6·3% (11/176). Dientamoeba fragilis frequency was higher among older children (21·9%), than younger children (2·8%), and greater in boys (6·8%) than in girls (5·5%). All positive children presented with diarrhoea associated with vomiting, fever and abdominal pain. Infection was associated with the age of children (P < 0·001), school attendance (P = 0·002) and consumption of certain foods (P = 0·014), e.g. cakes with crème and ham. The frequency of diantamoebiasis found in a cohort of hospitalized Portuguese children, with acute gastrointestinal disease, could be considered a very high value when compared with the protozoan frequency normally associated with this pathology. | pt_PT |
| dc.identifier.citation | Parasitology. 2015 Sep;142(11):1398-403. doi: 10.1017/S0031182015000906. Epub 2015 Jul 21 | pt_PT |
| dc.identifier.doi | 10.1017/S0031182015000906 | pt_PT |
| dc.identifier.issn | 0031-1820 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/3515 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Cambridge University Press | |
| dc.relation.publisherversion | http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9915506&fileId=S0031182015000906 | pt_PT |
| dc.subject | Dientamoeba fragilis | pt_PT |
| dc.subject | Acute Gastroenteritis | pt_PT |
| dc.subject | Frequency | pt_PT |
| dc.subject | Hospitalized Children | pt_PT |
| dc.subject | Portugal | pt_PT |
| dc.subject | Infecções Gastrointestinais | pt_PT |
| dc.title | Detection of Dientamoeba fragilis in Portuguese children with acute gastroenteritis between 2011 and 2013 | pt_PT |
| dc.title.alternative | Cambridge University Press | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1403 | pt_PT |
| oaire.citation.startPage | 1398 | pt_PT |
| oaire.citation.title | Parasitology | pt_PT |
| oaire.citation.volume | 142(11) | pt_PT |
| rcaap.rights | embargoedAccess | pt_PT |
| rcaap.type | article | pt_PT |