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Molecular characterization and antimicrobial susceptibility profiles in Streptococcus agalactiae colonizing strains: association of erythromycin resistance with subtype III-1 genetic clone family

2010-09Journal article Restricted access
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dc.contributor.authorFlorindo, C.
dc.contributor.authorViegas, S.
dc.contributor.authorPaulino, A.
dc.contributor.authorRodrigues, E.
dc.contributor.authorGomes, João Paulo
dc.contributor.authorBorrego, M. J.
dc.date.accessioned2011-09-19T12:16:10Z
dc.date.available2011-09-19T12:16:10Z
dc.date.issued2010-09
dc.description.abstractKnowledge of the epidemiology of Streptococcus agalactiae in Portugal is limited: therefore, the present study aimed to investigate the carriage rate of S. agalactiae among Portuguese women of reproductive age and the prevalence of antibiotic resistance, as well as to perform a molecular characterization of the clinical isolates. S. agalactiae was recovered from 6.2% of 4269 women during the period 2005–2007, with a predominance of capsular genotypes III (35%), V (33%), Ia (16%) and II (10%) in a sample of 100 isolates. To our knowledge, this is the first report of the S. agalactiae colonization rate in Portugal determined according to CDC guidelines. All isolates were susceptible to penicillin and vancomycin, whereas resistance to clindamycin and erythromycin was detected in 10% and 19% of isolates, respectively. Among the 19 erythromycin-resistant isolates, ten (53%) displayed the constitutive MLSB phenotype (conferring high-level resistance to macrolides), eight (42%) had the inducible MLSB, and the M phenotype accounted for one isolate (5%). erm methylase genes were exclusively associated with MLSB phenotype isolates, whereas the M phenotype was a result of the presence of mefA. Multilocus sequence typing analysis of the genetic relatedness among isolates presenting resistance to erythromycin demonstrated a novel association between erythromycin resistance and the subtype III-1/ST-19 genetic clone family.por
dc.identifier.citationClin Microbiol Infect. 2010 Sep;16(9):1458-63. Epub 2010 Feb 17por
dc.identifier.issn1198-743X
dc.identifier.otherdoi:10.1111/j.1469-0691.2010.03106.x
dc.identifier.urihttp://hdl.handle.net/10400.18/195
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2010.03106.x/abstract;jsessionid=75335F7204F0BD1D725F779369C7B7FD.d02t04por
dc.subjectAntibiotic resistancepor
dc.subjectCapsular genotypingpor
dc.subjectMLSTpor
dc.subjectPortugalpor
dc.subjectStreptococcus agalactiaepor
dc.subjectTransmissão verticalpor
dc.subjectInfecções Sexualmente Transmissíveispor
dc.titleMolecular characterization and antimicrobial susceptibility profiles in Streptococcus agalactiae colonizing strains: association of erythromycin resistance with subtype III-1 genetic clone familypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1463por
oaire.citation.startPage1458por
oaire.citation.titleClinical Microbiology and Infectionpor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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