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Validation of Caco-2/HT29-MTX model to assess the potential risk of ingested titanium dioxide nanoparticles

2020-09-12Conference object Restricted access
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dc.contributor.authorRolo, Dora
dc.contributor.authorPereira, Joana F.S.
dc.contributor.authorMatos, Paulo
dc.contributor.authorVieira, Adriana
dc.contributor.authorVital, Nádia
dc.contributor.authorJordan, Peter
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2020-10-28T17:39:58Z
dc.date.available2020-10-28T17:39:58Z
dc.date.issued2020-09-12
dc.description.abstractThe increased use of titanium dioxide nanoparticles (TiO2NPs) as a food additive demands a deep assessment of their potential risk for human health, including their abilities to cross biological barriers. In vitro models of the intestinal barrier are being increasingly used to evaluate NPs exposure risk. Most of these studies have focused on standard monoculture models of Caco-2 monolayers. However, they exhibit several limitations such as the lack of mucus layer and a low paracellular permeability. We aim to study TiO2NPs with an in vitro model of intestinal barrier using co-culture of two types of cells: absorptive Caco-2 and mucus-secreting HT29-MTX. This co-culture confers more physiological intestinal epithelium-like properties to the model, such as mucus secretion and tight junction formation, allowing a more adequate investigation of the cellular effects elicited by NPs. Due to the multiple variables and parameters playing a part when the model's complexity is increased, we characterized the robustness of this model by evaluating cell differentiation by confocal microscopy and Western blot while monitoring epithelial barrier formation, through measurement of both transepithelial resistance (TEER)and paracellular permeability (Lucifer yellow). An optimized model of the intestinal barrier will be used to better understand the uptake, adhesion and localization of TiO2NPs, both directly and after the simulation of the human digestive process using the harmonized in vitro digestion protocol. Preliminary data shows that these complex models can add valuable information to study the potential negative impacts and genotoxicity of TiO2NPs on human health.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through national funds (PIDDAC), PTDC/SAU-PUB/29481/2017 and co-funded by ToxOmics (UIDB/00009/2020)pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7227
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectintestinal Cellspt_PT
dc.subjectTitanium Dioxide Nanomaterialspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleValidation of Caco-2/HT29-MTX model to assess the potential risk of ingested titanium dioxide nanoparticlespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.citation.conferencePlace(Virtual meeting)pt_PT
oaire.citation.titleEnvironmental Mutagenesis & Genomics Society Virtual Annual Meeting (EMGS), 12-16 September 2020pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLouro
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication2361a951-8b9a-4b90-92d6-f6384003a242
relation.isAuthorOfPublication.latestForDiscovery2361a951-8b9a-4b90-92d6-f6384003a242
relation.isProjectOfPublication10a38358-808e-4126-a323-58973bebbcce
relation.isProjectOfPublicationa438b9d1-8a6a-4c90-a13b-7ccf34071451
relation.isProjectOfPublication.latestForDiscovery10a38358-808e-4126-a323-58973bebbcce

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