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In vitro combined toxicity of chromium, nickel and PAHS in human lung cells as support for mixtures risk assessment

2020-11-18Conference object Restricted access
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dc.contributor.authorAlves, Inês
dc.contributor.authorLadeira, Carina
dc.contributor.authorTavares, Ana M.
dc.contributor.authorViegas, Susana
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2021-03-19T15:50:07Z
dc.date.available2021-03-19T15:50:07Z
dc.date.issued2020-11-18
dc.description.abstractRisk assessment of chemicals mainly relies on exposure to single chemicals and their hazardous effects, although the real scenario in occupational settings is more commonly characterized by exposure to chemi-cal mixtures. Chromium (Cr), Nickel (Ni), and Polycyclic Aromatic Hydrocarbons (PAHs) are often pre-sent in occupational settings, such as aeronautic industries, where workers are exposed primarily through inhalation. Considering that those substances are recognized lung carcinogens acting mainly by genotoxic mechanisms, it is likely that interactive effects occur, indicating that the risk from occupational exposure to these chemicals should be assessed as a mixture. Such issue is being addressed in the HBM4EU Initiati-ve, where a real scenario of occupational exposure has been studied. In order to provide support to the hazard assessment of the referred mixture, the present work aimed to evaluate the combined toxicity of Cr(VI), Ni, and benzo(a)pyrene (BaP), using a human lung cell line (A549 cells). MTT assay was performed for each individual chemical and a dose-response curve was esta-blished, enabling the determination of the IC50. The combined toxicity of the Cr and Ni mixture as well as that of Cr, Ni and BaP were determined comparatively to the single chemicals’ toxicity to ascertain whet-her additive effects or deviations from additivity towards synergism or antagonism was obtained. The results will be presented and discussed and are expected to contribute to the overall mixture’s risk asses-sment.pt_PT
dc.description.sponsorshipFunded by the ChemMix (IDI&CA, IPL, 2019) and HBM4EU (GA 733032) projects and by FCT/MCTES through national funds (UIDB/00009/2020; UIDP/00009/2020).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7561
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation733032pt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectMixturespt_PT
dc.subjectRisk Assessmentpt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleIn vitro combined toxicity of chromium, nickel and PAHS in human lung cells as support for mixtures risk assessmentpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlaceLisboa, Portugal (online)pt_PT
oaire.citation.title3rd HBM-PT Workshop on Human BioMonitoring in Portugal, 18 november 2020pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLouro
person.familyNameSilva
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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