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Differential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansoni

2019-07-24Journal article Open access
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dc.contributor.authorNacif-Pimenta, Rafael
dc.contributor.authorda Silva Orfanó, Alessandra
dc.contributor.authorMosley, Ilana A.
dc.contributor.authorKarinshak, Shannon E.
dc.contributor.authorIshida, Kenji
dc.contributor.authorMann, Victoria H.
dc.contributor.authorCoelho, Paulo Marcos Zech
dc.contributor.authorda Costa, José M. Correia
dc.contributor.authorHsieh, Michael H.
dc.contributor.authorBrindley, Paul J.
dc.contributor.authorRinaldi, Gabriel
dc.date.accessioned2020-05-05T23:18:59Z
dc.date.available2020-05-05T23:18:59Z
dc.date.issued2019-07-24
dc.description.abstractChronic urogenital schistosomiasis can lead to squamous cell carcinoma of the bladder. The International Agency for Research on Cancer classifies the infection with S. haematobium as a group 1 carcinogen, a definitive cause of cancer. By contrast, hepatointestinal schistosomiasis due to the chronic infection with S. mansoni or S. japonicum associated with liver periportal fibrosis, does not apparently lead to malignancy. The effects of culturing human epithelial cells, HCV29, established from normal urothelium, and H69, established from cholangiocytes, in the presence of S. haematobium or S. mansoni eggs were investigated. Cell growth of cells co-cultured with schistosome eggs was monitored in real time, and gene expression analysis of oncogenesis, epithelial to mesenchymal transition and apoptosis pathways was undertaken. Schistosome eggs promoted proliferation of the urothelial cells but inhibited growth of cholangiocytes. In addition, the tumor suppressor P53 pathway was significantly downregulated when exposed to schistosome eggs, and downregulation of estrogen receptor was predicted in urothelial cells exposed only to S. haematobium eggs. Overall, cell proliferative responses were influenced by both the tissue origin of the epithelial cells and the schistosome species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSci Rep. 2019 Jul 24;9(1):10731. doi: 10.1038/s41598-019-46917-ypt_PT
dc.identifier.doi10.1038/s41598-019-46917-ypt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.18/6600
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-019-46917-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectChronic Urogenital Schistosomiasispt_PT
dc.subjectEpithelial Cellspt_PT
dc.subjectUrinary Tractpt_PT
dc.subjectBiliary Tractpt_PT
dc.subjectSchistosoma haematobiumpt_PT
dc.subjectS. mansonipt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleDifferential responses of epithelial cells from urinary and biliary tract to eggs of Schistosoma haematobium and S. mansonipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage10731pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume9pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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