Adenoids, friends or foes?

Subtil, João; Lavado, Paula; Rodrigues, João; Rodrigues, Lúcia; Nogueira, Isabel; Duarte, Aida; Jordao, Luisa

Publication

Adenoids, friends or foes?

2017-09Conference object

dc.contributor.author	Subtil, João
dc.contributor.author	Lavado, Paula
dc.contributor.author	Rodrigues, João
dc.contributor.author	Rodrigues, Lúcia
dc.contributor.author	Nogueira, Isabel
dc.contributor.author	Duarte, Aida
dc.contributor.author	Jordao, Luisa
dc.date.accessioned	2018-02-14T16:15:20Z
dc.date.available	2018-02-14T16:15:20Z
dc.date.issued	2017-09
dc.description.abstract	The main goal of this study is to evaluate the existence of a link between biofilm assembly on adenoids and the incidence of recurrent infections within a paediatric population. Thirty-three different bacterial genera were isolated from 186 samples (nasal/adenoid scrubs and adenoid biopsies) being Haemophilus, Neisseria, Streptococcus and Staphylococcus the most frequent. Biofilms were present in 27.4% of the adenoid samples as assessed by SEM. Since the aim of the study is to assess the role of biofilms on adenoid colonization/ invasion and onset of infection a group of samples harbouring clinically relevant bacteria (H.influenzae, S. aureus, S. pyogenes, S. pneumococcus and Moraxella) both on the adenoid surface and core were selected for further studies. Based on biofilm assembly in vitro, assessed by crystal violet assay, bacteria were classified as weak, moderate and strong biofilm assemblers. No direct relation between the ability to assemble biofilms in vitro and the presence of biofilms on the adenoid (biofilm in vivo) was found. A similar result was obtained for antibiotic susceptibility with the majority of bacteria being antibiotic susceptible independently of its origin (sample with or without biofilms). This result might be explained at least partially, by the nature of the sample since the adenoidectomy can only be performed in individuals without infection. H. influenzae, the most isolated bacterium, is an opportunistic pathogen, highly adapted to colonize the upper respiratory tract and easily progresses to infection, especially in children. For this reason, virulence factors such as the capsular type were investigated by PCR. However, all strains were characterized as non-capsulated, which might explain adenoid colonization and biofilm formation, as have been also described in the literature. Further studies must be performed to validate the thesis that adenoids function as a reservoir of etiologic agents of respiratory and ear infections.	pt_PT
dc.description.sponsorship	INSA - financial support (Grant 2015DDI1143) to research physicians Luís Freitas, Joana Filipe, Alberto Santos and Carlos Macor.	pt_PT
dc.description.version	N/A	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.18/4956
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	pt_PT
dc.subject	Biofilmes	pt_PT
dc.subject	Adenoides	pt_PT
dc.subject	Paediatric Population	pt_PT
dc.subject	Infectious Diseases	pt_PT
dc.subject	Infecções Respiratórias
dc.title	Adenoids, friends or foes?	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.citation.conferencePlace	Amsterdão, The Netherlands	pt_PT
oaire.citation.title	5th Eurobiofilms congress, 19-22 September 2017	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	conferenceObject	pt_PT