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How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial

2023-03Journal article Open access
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dc.contributor.authorSantos, Hugo M.
dc.contributor.authorCarvalho, Luís B.
dc.contributor.authorLodeiro, Carlos
dc.contributor.authorMartins, Gonçalo
dc.contributor.authorGomes, Inês L.
dc.contributor.authorAntunes, Wilson D.T.
dc.contributor.authorCorreia, Vanessa
dc.contributor.authorAlmeida-Santos, Maria M.
dc.contributor.authorRebelo-de-Andrade, Helena
dc.contributor.authorMatos, António P.A.
dc.contributor.authorCapelo, J.L.
dc.date.accessioned2024-01-18T14:22:41Z
dc.date.available2024-01-18T14:22:41Z
dc.date.issued2023-03
dc.description.abstractThe capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.pt_PT
dc.description.abstractHighlights: - Immunohistochemistry with magnetic core nanoparticles to isolate viruses. - The use of MALDI-MS for rapid virus detection is explained in detail; - The use of ESI-MS/MS to pinpoint host-patient crosstalk is explained in detail. - The absolute quantitative MS is explained for large-scale protein quantitation.pt_PT
dc.description.sponsorshipThis work received financial support from PT national funds (FCT/MCTES) through the projects UIDB/50006/2020 and UIDP/50006/2020 and from PROTEOMASS Scientific Society through the projects #PM001/2019 and #PM003/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicrochem J. 2023 Mar;186:108323. doi:10.1016/j.microc.2022.108323. Epub 2022 Dec16pt_PT
dc.identifier.doi10.1016/j.microc.2022.108323pt_PT
dc.identifier.issn0026-265X
dc.identifier.urihttp://hdl.handle.net/10400.18/8930
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0026265X22011511pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectImmuno-mass Spectrometrypt_PT
dc.subjectViral Detectionpt_PT
dc.subjectMALDI-TOF-MSpt_PT
dc.subjectLC-ESI-Q-TOF-MS/MSpt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.titleHow to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.citation.startPage108323pt_PT
oaire.citation.titleMicrochemical Journalpt_PT
oaire.citation.volume186pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com a página web da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationeccce5f3-585c-46ef-9277-7db5962d45f7
relation.isProjectOfPublication226cfaf5-2eca-4b13-b46b-2f1c376c8172
relation.isProjectOfPublication.latestForDiscoveryeccce5f3-585c-46ef-9277-7db5962d45f7

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