Repository logo
 
Publication

Effects of physical exercise training in DNA damage and repair activity in humans with different genetic polymorphisms ofhOGG1(Ser326Cys)

2015-12Journal article Restricted access
Simple item page
dc.contributor.authorSoares, Jorge Pinto
dc.contributor.authorSilva, Ana Inês
dc.contributor.authorSilva, Amélia M.
dc.contributor.authorAlmeida, Vanessa
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorMatos, Manuela
dc.contributor.authorGaivão, Isabel
dc.contributor.authorMota, Maria Paula
dc.date.accessioned2016-10-19T16:10:21Z
dc.date.available2016-12-01T01:30:08Z
dc.date.issued2015-12
dc.description.abstractThe main purpose of this pilot study was to investigate the possible influence of genetic polymorphisms of the hOGG1 (Ser326Cys) gene in DNA damage and repair activity by 8-oxoguanine DNA glycosylase 1 (OGG1 enzyme) in response to 16 weeks of combined physical exercise training. Thirty-two healthy Caucasian men (40-74 years old) were enrolled in this study. All the subjects were submitted to a training of 16 weeks of combined physical exercise. The subjects with Ser/Ser genotype were considered as wild-type group (WTG), and Ser/Cys and Cys/Cys genotype were analysed together as mutant group (MG). We used comet assay in conjunction with formamidopyrimidine DNA glycoslyase (FPG) to analyse both strand breaks and FPG-sensitive sites. DNA repair activity were also analysed with the comet assay technique. Our results showed no differences between DNA damage (both strand breaks and FPG-sensitive sites) and repair activity (OGG1) between genotype groups (in the pre-training condition). Regarding the possible influence of genotype in the response to 16 weeks of physical exercise training, the results revealed a decrease in DNA strand breaks in both groups, a decrease in FPG-sensitive sites and an increase in total antioxidant capacity in the WTG, but no changes were found in MG. No significant changes in DNA repair activity was observed in both genotype groups with physical exercise training. This preliminary study suggests the possibility of different responses in DNA damage to the physical exercise training, considering the hOGG1 Ser326Cys polymorphism.pt_PT
dc.description.sponsorshipThis work was supported by the Foundation of Science and Technology (FCT) for the research grant SFRH/BD/66438/2009 to J. S. and for the project entitled ‘Physical exercise role on human lymphocyte DNA damage reduction: possible influence of oxidative stress and DNA repair capacity’ PTDC/DES/121575/2010. We also would like to acknowledge FCT and FEDER/COMPETE under the references PEst-C/AGR/UI4033/2014.pt_PT
dc.identifier.citationCell Biochem Funct. 2015 Dec;33(8):519-24. doi: 10.1002/cbf.3134. Epub 2015 Nov 22.pt_PT
dc.identifier.doi10.1002/cbf.3134pt_PT
dc.identifier.issn0263-6484
dc.identifier.urihttp://hdl.handle.net/10400.18/4058
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley and Sonspt_PT
dc.relationPhysical exercise role on Human` lymphocyte DNA damage reduction: possible influence of oxidative stress and DNA repair capacity
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/cbf.3134/pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectPhysical Exercise Trainingpt_PT
dc.subjecthOGG1 Polymorphismpt_PT
dc.subjectDNA damagept_PT
dc.subjectDNA Repair Activitypt_PT
dc.subjectStrengthpt_PT
dc.subjectAerobic Capacitypt_PT
dc.subjectToxicologiapt_PT
dc.titleEffects of physical exercise training in DNA damage and repair activity in humans with different genetic polymorphisms ofhOGG1(Ser326Cys)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePhysical exercise role on Human` lymphocyte DNA damage reduction: possible influence of oxidative stress and DNA repair capacity
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FDES%2F121575%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/Incentivo%2FAGR%2FUI4033%2F2014/PT
oaire.citation.endPage524pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage519pt_PT
oaire.citation.titleCell Biochemistry and Functionpt_PT
oaire.citation.volume33(8)pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1b035030-e8a7-423c-8071-c4124e68c2e7
relation.isProjectOfPublicationb77e0ea8-17e6-4415-bb07-9392cade1faf
relation.isProjectOfPublication.latestForDiscoveryb77e0ea8-17e6-4415-bb07-9392cade1faf

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Effects of physical exercise training in DNA damage and repair activity in humans with different genetic polymorphisms of hOGG1 (Ser326Cys).pdf
Size:
232.97 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DSA - Artigos em revistas internacionais